1 Jaw., 1899.] QUEENSLAND AGRICULTURAL JOURNAL. 65 
6. 
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12) 
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(a) No more blood should be taken from an animal and defibrinated 
than can be used within six hours, for if kept longer the blood is 
apt to become contaminated with various kinds of bacteria, which 
are floating about in the atmosphere of the stockyard or shed, or 
other foreign matter, which, injected into an animal, might either 
produce blood-poisoning or an abscess. 
(b) For general inoculation purposes, only use the blood from an 
animal that has given a decided reaction after an injection of 
either recovered or virulent blood. 
. Bive ec. of blood has been found to be a standard dose for 
animals of any age and either sex. 
Inoculation should proceed steadily, care being taken to avoid all 
attempts at breaking records. 
. During hot summer weather inoculate only early in the morning and 
late in the afternoon. 
(a) Never inoculate cows heavy in caif. 
(b) It should be remembered that old bulls are the most susceptible 
of all animals; therefore, if there is no immediate danger from 
natural tick infection, it is inadvisable to inoculate them. 
It is advisable, when inoculating on an extensive scale, to occa- 
sionally examino and test the needle and syringe, in order to see 
that they work freely, as a little piece of dirt or a hair will throw 
the valves out of order. 
Cattle should be inoculated as near to their own pastures as 
possible, and in any case should, if inoculated some distance away, 
be allowed to travel back slowly without the aid of whips and dogs. 
. After inoculation, cattle should not be disturbed or interfered with 
in any way for at least three weeks. 
. During the cold weather in winter months it has been proved that 
blood can be carried with safety in well-corked clean bottles for 
from twenty-four to thirty-six hours either by rail, coach, or on 
horseback. 
. All the inoculating instruments, before and after use, should be 
thoroughly disinfected with 1 in 20 carbolic acid solution. 
The rubber-washers and plunger of the piston-rod should be 
thoroughly cleaned and washed in carbolic solution, and a little 
‘glycerine applied, which will prevent the rubber from becoming 
hard and deteriorating. 
It is advisable in the latest form of syringe to soak the red rubber 
washers in warm water before use. 
Never use vaseline or oil of any description on the rubber parts of 
the syringe. Glycerine is the best lubricant. 
The needles and trocar after use should be well washed in boiled 
water, then rinsed in carbolic acid (1. in 20) solution, carefully 
dried, and rubbed all over with a little vaseline. 
Never disinfect any of the inoculating instruments with corrosive 
sublimate (bichloride of mercury) solution, as it readily corrodes 
the nickel plating. 
The long pieces of rubber tubing when not in use should be kept 
under water, to which a few crystals of thymol have been added, in 
a large, well-stoppered, wide-mouth jar, 
Important Note.—it should be specially pointed out that repeated 
experiments have shown that some animals do not react to the first inoculation ; 
therefore it is recommended that, wherever practicable, cattle be inoculated a 
second time after an interval of not less than six weeks, for experience tends 
to prove that a second inoculation rarely ever fails. Moreover, by following 
this mode of procedure, stockowners have the satisfaction of knowing that the 
possibility of their cattle not being immune to a subsequent attack of natural 
Tick Hever is reduced to a minimum. 
E 
