BULLETIN OF THE BUSSEY INSTITUTION. . 67 
second heavy, white precipitate was obtained. It was decom- 
posed with carbonic acid as before, and the solution was treated 
and tested in the same way as that obtained from the first pre- 
cipitate had been treated. Some crystals of dextrose-osazone, 
but no indications of mannose-hydrazone were noticed. Yet 
another, third, precipitate obtained by means of the basic lead 
acetate was treated in the same way as the preceding, but no 
mannose-hydrazone was observed. 
The three precipitates produced on the addition of the phenyl- 
hydrazin acetate reagent, as above stated, were washed with 
water, then put together and washed with a few drops of glacial 
acetic acid and recrystallized from warm diluted alcohol (3:1), 
but no indications of mannose-hydrazone were detected. 
On the further addition of basic lead acetate to the filtrate from 
the third precipitate above mentioned, a fourth and a fifth pre- 
cipitate were obtained. They were treated as the earlier precipi- 
tates had been treated, but no indications of mannose-hydrazone 
were seen in either instance. The five precipitates, consisting of 
lead carbonate, etc., as left after passing carbonic acid through 
the precipitates produced by basic lead acetate were ground in a 
mortar, together with dilute sulphuric acid, the lead sulphate was 
filtered off and to the filtrate sodium hydroxide was added to slight 
alkalinity. The liquid was evaporated to soft dryness, and the 
residue treated with warm diluted alcohol. The filtered alcoholic 
solution was evaporated to dryness, the residue was taken up 
with 3 cc. of water, and 16 drops of the phenylhydrazin acetate 
reagent were added. No indications of mannose-hydrazone were 
obtained. 
Finally, the matter left in the can from which the molasses 
had been poured was dissolved in 250 cc. of water, and 2 or 3 cc. 
of the basic lead acetate solution were added. ‘The heavy, white 
gelatinous precipitate was washed with water and decomposed 
with carbonic acid as before. The filtrate from the lead carbonate 
was evaporated to a thick syrup which was treated with diluted 
alcohol. The alcoholic filtrate was evaporated in its turn to a 
thick syrup which was taken up with 3 cc. of water; 16 drops of 
the phenylhydrazin acetate solution were added to this aqueous 
solution. There were no indications of mannose-hydrazone. <A 
second white precipitate, obtained by adding basic lead acetate 
