BULLETIN OF THE BUSSEY INSTITUTION. 69 
No. 4.— A supplement to the article (No. 3) on the Occur- 
rence of Mannan in Trees, foots, and Fruit.* By F. H. 
SroreER, Professor of Agricultural Chemistry. 
Pine wood cut in June.— To complete the series of experiments on the 
quantity of mannan in white pine wood at different times in the year, 
as set forth in the tabular statement on page 50, a limb 14 inches in 
diameter was cut from a thrifty young pine tree at Hingham, Mass., on 
June 9, 19038, and 22 grm. of the wood were subjected to hydrolysis 
with hydrochloric acid of 5 per cent. The product of the hydrolysis 
was treated and tested in the same way that the other specimens men- 
tioned in the table had been treated. In this case, crystals of mannose- 
hydrazone were obtained, on adding phenylhydrazin acetate, in 1 ce. 
of the unevaporated product of the hydrolysis, but not in 1 cc. samples 
that were diluted respectively with 1, 2, and 3 cc. of water before 
adding the phenylhydrazin reagent. 
After the remainder of the product of the hydrolysis had been neutralized 
and evaporated to dryness and the residue taken up with 10 ce. of 
water tests with phenylhydrazin acetate applled to 1 cc. of this solu- 
tion, without further dilution, and to 1 cc. portions that were diluted 
respectively with 1, 2, and 3 cc. of water, all gave balls of mannose- 
hydrazone, but none of this substance was seen in trials where 1 ce. 
portions of the solution were diluted with 4 cc. and 5 cc. of water. 
Pine wood that had long lain buried in a swamp.— The sample hydro- 
lyzed was a chip cut from a large log three feet in diameter that had 
been dug up in Bear Swamp, Hingham, Mass., a few years pre- 
viously. Mr. EH. Hersey, to whose kindness I am much indebted, 
assures me that the tree must have fallen more than two hundred years 
ago and buried itself so deeply in the bog that the wood was preserved 
from decay. To all appearance the wood was white pine, though Mr. 
Hersey suggests that the tree may possibly have been an example of a 
somewhat apocryphal ‘‘ yellow pine” that is thought by some to have 
grown formerly in Massachusetts and to have been neither pitch pine 
(Pinus rigida) nor red (‘‘ Norway”) pine (Pinus resinosa). 
After hydrolyzing with hydrochloric acid of 5 per cent., no crystals or balls 
of mannose-hydrazone were got from the unevaporated product of the 
hydrolysis. But after evaporation mannose-hydrazone was obtained 
from 1 cc. of the undiluted solution, and also from 1 cc. that was 
diluted with 1 cc. of water; but no mannose-hydrazone was obtained 
from portions that were more strongly diluted. 
* Bulletin of the Bussey Institution, 1903, 3.47. It may here be said 
that the methods of analysis and procedure adopted in this supplementary 
note are precisely the same as those employed in the original article (No. 3), 
unless otherwise expressly stated. 
