34 BULLETIN OF THE BUSSEY INSTITUTION. 
4.5 inches in diameter, felled respectively on May 1 and on May 30 at West 
Newfield, Me., and also that doubtful traces were noticed in the wood of a 
root 1 to 1.5 inches in diameter of a sugar-maple dug up in November at 
West Newfield, though no indications of mannose were seen on testing the 
unevaporated liquors. 
5. That no mannose was detected in the unevaporated product of the 
hydrolysis 
of small white beans (Phaseolus) ; or 
of the small terminal twigs of a gray birch (Betula popultfolia), col- 
lected November 2 at the Bussey Institution, from a tree 3 or 4 
inches in diameter and some 15 feet high. 
In neither of these instances was the evaporated liquor tested. 
6. That no traces of mannose could be discovered either in the evaporated 
or the unevaporated liquors from the hydrolysis ; 
of the inner trunk-wood of the sugar-maple, felled on May 30; 
of the trunk-wood of several gray birches (Betula popultfolia), 
- felled at different times and seasons, but especially in the autumn 
and winter; 
of the seeds of the gray birch; 
of the wood of a limb, 4.5 inches in diameter, cut off from a willow 
tree (Saliz alba) on January 10, at the Bussey Institution ; 
of the trunk-wood of a poplar tree (P. tremuliformis), 4 inches -in 
diameter, felled December 14 at Hingham, Mass. ; 
of the trunk-wood of the so-called leather wood [or moose wood] 
(Dirca palustris) one inch in diameter, procured on April 11 
at West Newfield, Me., — no starch grains could be detected with 
the microscope in this wood; 
of orange seeds; 
of peas; 
of the root of the yellow pond lily (Beaver root) (Nuphar advena). 
Since an abundance of crystalline osazone balls (‘‘sea-urchins”) were 
obtained from the product of the hydrolysis of the lily root, it is evident that 
there is contained in it some matter (presumably cellulose) capable of yield- 
ing dextrose or some other kind of sugar. 
The points of chief interest in the foregoing enumeration of 
tests are :— 
1. The presence of mannan in the trunk-wood of sugar maple 
trees, especially in the wood of trees felled during the period of 
hibernation, — that is, in January and October, —and the com- 
paratively small quantities detected in maple trees felled at the 
beginning and the end of May,—that is, during or after the 
formation of new suits of leaves upon the trees. I have insisted 
in another place* on the improbability of the popular opinion that 
* Bulletin of the Bussey Institution, 1900, 2. 437; also 1897, 2. 386 et seq. 
