BULLETIN OF THE BUSSEY INSTITUTION. 33 
3. That good evidence of the presence of mannose has been seen on testing 
the evaporated product of the hydrolysis 
of the wood of the limb of the sugar-maple, 2.25 inches in diameter, 
cut off on January 21; 
of the outer and to a less extent of the inner trunk-wood of a sugar- 
maple tree, 6 inches in diameter, felled on October 17 at West 
Newfield, Me. ; 
of the seeds of the white pine; 
of the seeds of the Norway spruce ; 
of the seeds of the horse chestnut (Aesculus hippocastaneumy) ; 
of the small twigs on the limb of the Japanese larch, above mentioned ; 
of the seeds of the red clover; 
of orange peel; 
of the root of a Jerusalem artichoke (Helianthus tuberosus) ; 
of two samples of the roots of chicory plants (Cicoriwm intybus) 
dug up at the Bussey Institution, one in late September and the 
other on May 6, before flower stalks had begun to develop; 
of dandelion roots (Taraxacum officinale) dug up at the Bussey 
Institution on May 6, when the plants were on the point of blos- 
soming ; 
of the roots of asparagus plants (Asparagus officinalis) dug up on 
October 3, at Hingham, Mass. 
No mannose was detected in the unevaporated products of the hydrolysis 
of either of the substances in this list, with the exception of those from the 
maple wood felled in January, the red clover seed, and the orange peel, and 
it was noticed that much stronger reactions for mannose were obtained from 
the evaporated products of the hydrolyses of these three substances than had 
been got previously on testing the unevaporated liquors. 
The great mass of soft vegetable matter and of water contained in the 
Jerusalem artichoke made it necessary to proceed with special care in testing 
for mannose in the products of the hydrolysis. In one case where 20 grm. 
of the natural, undried root, taken from the ground in late November, were 
hydrolyzed with 200 cc. of 5 per cent. hydrochloric acid, excellent crystals of 
mannose-hydrazone were obtained on testing 1 cc. portions of the evaporated 
liquor. But no satisfactory result was got in a subsequent trial where 20 grm. 
of the crushed root were left in contact with an equal quantity of concen- 
trated sulphuric acid during thirty-six hours, and the mixture was boiled for 
three hours after the acid has been diluted with water to a strength of about 
5 per cent. (1.033 sp. gr.). After neutralizing with dilute caustic soda, 
evaporating to dryness and taking up with 10 cc. of water, no mannose- 
hydrazone was detected. But on drying a quantity of the root and treating 
20 grm. of the dry powder with sulphuric acid in the same way that the 
natural root had been treated in the foregoing experiment, there was no diffi- 
culty in obtaining crystals of mannose-hydrazone on testing the liquor after 
it had been neutralized with marble dust and evaporated to dryness. 
4. That traces of mannose were detected on testing the solutions taken up 
from the evaporated product of the outer trunk-wood of sugar maples, 5 and 
