BULLETIN OF THE BUSSEY INSTITUTION. yal | 
dextrose was applied to several of the solutions used in the fore- 
going series of tests. Thus, 1 cc. of the dextrose solution 
(0.2 grm. in 5 cc. water) was added to a mixture of five drops 
of phenylhydrazin, ten drops of glacial acetic acid, and 1 ce. of 
a saturated solution of sodium chloride and boiled during two 
minutes. A heavy, canary-yellow precipitate fell, in which could 
be seen many pale green needles, like pine needles, as well as 
tufts of needles and masses of fan-like needles radiating from 
central points. On recrystallizing from warm diluted alcohol, 
still finer and more delicate needles were obtained; and on dilut- 
ing the dextrose solution with twice its volume of water and 
again applying the clinical test, still finer crystals were to be 
seen. There could be no question as to their similarity with the 
crystals formed, as above stated, in mixtures of dextrose and 
phenylhydrazin compounds left to stand for some time at the 
ordinary temperature of the air. 
These clinical tests were repeated in solutions made by mixing 
1 ce. of pure dextrose solution with 2 cc. of healthy urine. The 
precipitates were seen to contain the same small, green, radiating 
needles and spiny balls of dextrose-osazone, as before, though 
the precipitate did not have the bright yellow color of that ob- 
tained in the absence of the urine. So, too, when mixtures of 
*¢olucose ” and urine were treated. Many of the figures given 
by Pavy * well represent the osazone crystals obtained in these 
trials. 
In several instances the clinical test for dextrose was applied 
also to products of the hydrolysis of the ivory nut, by adding 
phenylhydrazin, glacial acetic acid, and a solution of sodium 
chloride to a few drops of the liquor, and boiling for two minutes. 
The yellow precipitates obtained showed usually, under the micro- 
scope, mixtures of globules and rosettes of mannose-hydrazone 
commingled with the needles, sharp-pointed spiny balls, and brush- 
like radiations of dextrose-osazone. 
* Physiology of the Carbohydrates, London, 1894; notably the figure on 
page 51. 
