12 BULLETIN OF THE BUSSEY INSTITUTION. 
Figs. — Dried and pressed figs of commerce, bought May 29, 1903. After 
having been cut into small pieces and dried for several days, the frag- 
ments were cut into very small pieces, 22 grm. of which were rubbed. 
up in a mortar together with enough hydrochloric acid of 5 per cent. 
to make a thin paste, which was poured into a flask and made up to the 
volume of 220 cc. by adding more of the acid. The mixture was 
boiled during three hours. Ten cc, of the clear filtrate were treated 
with sodium hydroxide to slight alkalinity, and then made faintly acid 
with hydrochloric acid. On adding the phenylhydrazin acetate reagent. 
to 1 ec. of this solution excellent crystals of mannose-hydrazone were 
obtained, but none were got when 1 cc. portions of the solution were 
diluted with 1 and 2 cc. of water before adding the phenylhydrazin 
acetate. When the product of the hydrolysis was evaporated to soft. . 
dryness and the residue was taken up with 10 cc. of water, good 
crystals of mannose-hydrazone were obtained on adding the phenyl- 
hydrazin acetate to 1 cc. of the solution, and to 1 ce. portions which 
were diluted respectively with 1 and 2 cc. of water, but not when 
1 ec. portions were diluted with 3 and 4 cc. of water. 
Carrot Leaves. — Plucked on October 19, 1908. The leaves were dried at 
a moderate heat and ground to powder. No mannose-hydrazone was. 
detected either in the unevaporated or the evaporated product of the 
hydrolysis. 
June Grass (Poa pratensis).— Fresh, young leaves of the grass mown on. 
May 6, 1904, when they were four inches long. The fresh grass was 
cut into small pieces, which were hydrolyzed directly with hydrochloric 
acid of 5 per cent. No mannose-hydrazone was detected either in the: 
unevaporated or the evaporated product of the hydrolysis. 
Oat Hay.— The oat plants were mown on the Bussey Farm towards the 
end of June, 1902, when the grain was ‘‘in the milk.” The hay had 
been stored in a dry barn until November, 1903. As a preparation 
for the hydrolysis the hay was cut into small pieces and ground to a. 
fine meal. No mannose-hydrazone was detected either in the unevapo-- 
rated or the evaporated product of the hydrolysis. 
Hydrolysis of Ivory Nut and Birch Wood with Nitric Acid. — 
For the sake of seeing what happens when ligneous matters that: 
do or do not contain mannan are hydrolyzed with dilute nitric 
acid a couple of experiments were made, one with the ivory nut 
(Phytelephas macrocarpa) and one with the wood of the gray 
birch (Betula populifolia). 
Clean buttons cut from the ivory nut were ground to a fine 
powder, and 22 grm. of this powder were boiled during three 
hours with 220 cc. of nitric acid of 5 per cent. ‘Ten ee. of the 
filtered product of the hydrolysis were neutralized with sodium 
hydroxide to slight alkalinity, and were then made faintly acid. 
