BULLETIN OF THE BUSSEY INSTITUTION. 13 
with nitric acid. To 1 cc. of this solution 12 drops of the 
phenylhydrazin acetate were added. A yellow precipitate formed 
slowly. After standing for one hour it was examined with the 
microscope, and was seen to contain many mannose-hydrazone 
crystals as well as many osazone crystals with needle-like spines. 
On recrystallizing from 3:1 alcohol, there was obtained excellent 
clean cut crystals of mannose-hydrazone in the form of plates 
and prisms. 
In a precisely similar trial made with nitric acid and wood of 
the gray birch, instead of the ivory nut, no mannose-hydrazone 
was detected either in the ‘unevaporated or the evaporated product 
of the hydrolysis. Crystals of osazones were seen, naturally 
enough. 
As was the case in the experiments to which this article forms 
a supplement, I am indebted to my assistant, Mr. F. T. Dilling- 
ham, for zealous aid in this research. 
