BULLETIN OF THE BUSSEY INSTITUTION. 107 
Tests for Mannite in Clover and Vegetables. 
Clover.— No mannite was detected in leaves of red clover collected on 
September 28, 1903, neither in the fresh leaves nor in those dried slowly in 
the shade; nor was any mannite found in the fresh leaves, flower stalks, 
or blossoms of red clover collected on June 23, 1904, when the plants were 
passing out of bloom. The several parts of the plants were examined 
separately. 
- Onions. — No mannite was detected in new onions bought on September 15, 
1904. After removal of the outer brown skins, the flesh of the onions was 
chopped fine and 300 grm. of it were subjected to hot water percolation and 
subsequent treatment in the usual way. 
Cabbage. — No mannite was detected in a head of cabbage bought on 
March 11, 1904. The ground up cabbage, mixed with sand, clogged the 
percolator and had to be extracted by way of decantation, using repeated small 
quantities of boiling water and filtering through a tuft of cotton finally with 
the help of a filter pump. 
Carrots. — No mannite was detected in the flesh of a yellow carrot bought 
on April 7, 1904. The material could not be percolated.. It was extracted 
with boiling water, by way of decantation. 
Parsnips. — No mannite was detected in the flesh of a parsnip bought on 
March 5, 1904. ‘The material clogged the percolator and had to be extracted 
by way of decantation. 
Turnips. — In the flesh of a yellow turnip bought March 17, 1904, mannite 
was readily detected. The material could not be percolated but was extracted 
with boiling water. After treatment with ammoniacal copper sulphate in the 
usual way, and thereafter with ferrous sulphate and hydrogen dioxide, and 
finally with phenylhydrazin acetate, large balls of mannose-hydrazone were 
obtained which gave good crystals on being recrystallized from 3: 1 alcohol. 
Celery Leaves.— No mannite was detected in the green leaves cut from 
the tips of celery stalks bought on November 21, 1903, January 14, 1904, and 
March 8, 1904. The three samples were dried and ground up together to a 
fine meal, which had to be leached by way of decantation. 
Carrot Leaves.— Leaves of the white Belgian carrot were gathered on 
October 19, 1903, at the Bussey farm and allowed to dry until January 7, 
1904. The powdered leaves were percolated with boiling water and the solu- 
tion was precipitated with ammoniacal copper sulphate, in the usual way. A 
small quantity of crystals that appeared to be mannite were obtained on 
evaporating the filtrate from the copper sulphide, and on being treated with 
ferrous sulphate and hydrogen dioxide and with phenylhydrazin acetate they 
gave mannose-hydrazone balls. It was evident that the dried carrot leaves 
contained some mannite. 
