126 BULLETIN OF THE BUSSEY INSTITUTION. 
ten times as many cubic centimeters of hydrochloric acid of five 
per cent. as there had been taken grams of the air-dried material. 
The contents of the flask were filtered after having stood over 
night, the residue was washed with ten cubic centimeters of 
water, and the washings were added to the filtrate. Ten cubic 
centimeters of the liquid were neutralized to slight alkalinity with 
a solution of sodium hydroxide (1-8), and then rendered faintly 
acid with a few drops of a 5 per cent. solution of hydrochloric 
acid. This unevaporated liquid was next divided into several 
one cubic centimeter portions. One of these portions was tested 
directly by the addition to it of twelve drops of a solution of 
‘phenyl-hydrazine acetate. [This reagent was prepared by adding 
2 c.c. of glacial acetic acid to 1 c.c. of phenyl-hydrazine, and 
shaking thoroughly after the addition of 10 c.c. of water.] Other 
one cubic centimeter portions of the liquid were diluted with one 
cubic centimeter and two cubic centimeters of water, respec- 
tively, and to each of these diluted portions twelve drops of the 
solution of phenyl-hydrazine acetate were added. The precipi- 
tates which formed were examined under the microscope, but in 
no case was mannose-hydrazone detected in any of these unevap- 
orated liquids. 
The remainder of the filtrate (and washings) from hydrolysis 
was neutralized to slight alkalinity with a solution of sodium 
hydroxide (1-8), and then made faintly acid by the addition of a 
small quantity of a 5 per cent. solution of hydrochloric acid. 
The nearly neutral liquid was evaporated on a water-bath to soft 
dryness. The residue was warmed with ten cubic centimeters of 
water, allowed to cool, and the liquid filtered. ‘The residue was 
washed with one cubic centimeter of water; the washings were 
added to the filtrate and thoroughly mixed with it. This evapo- 
rated liquor was tested in the following manner: It was divided 
into several one cubic centimeter portions. To one of these por- 
tions sixteen drops of a solution of phenyl-hydrazine acetate were 
added directly. Other one cubic centimeter portions were diluted 
with one, two, and three cubic centimeters of water, respectively, 
and to each of these diluted portions sixteen drops of the solution 
of phenyl-hydrazine acetate were added. The precipitates which 
formed were examined under the microscope within two hours 
after they had fallen, and again on the next day. If no mannose- 
