134 ISABURO NAGAT: 
Reagent, No, of hours Material. Germinated. | Not germ. Total. 
(Aqueous sol.) steeping. 
K2.0r207210% 24 YeD: 0 5 5 
5 Wire: 0 5 5 
” Ne an 1 4 5 
Cr, 0, 10% 24 Woe 1DY 0 > 5 
Wen. 0 5 > 
= NER. 0 5 | 5 
The more important points brought out in the preceding tables are 
as follows : 
(1) The desiccated grains of Oryza and Zea are far more resistant than 
air dried ones. To the air dried Oryza grain twenty four hours’ steeping in 
chloroform, acetone, commercial absolute ethyl alcohol, picric acid (saturated 
solution), absolute ethyl alcoholic solution of thymol, naphthalene, c-naphthol, 
and twenty one hours’ steeping in 6N sulphuric acid are fatal, whereas the 
same treatment on the desiccated grain is only slightly noxious or entirely 
harmless. Likewise in the case with Zea, commercial absolute ethyl alcohol, 
the absolute ethyl alcohol solution of naphthalene (1%), resorein (5%), a- 
naphthol (5%), «-naphthylamine (10%), 6 N sulphuric acid and hydrochloric 
acid are fatal to the air dried grain, but not to the desiccated one. 
(2) The following treatment is proved to be fatal both to desiccated 
and air-dried grains of Oryza and Zea: steeping them for twenty four hours 
in commercial absolute methyl alcohol, formaldehyde, formic acid, methyl ether, 
chloralhydrate (10% aqueous solution), acetaldehyde, acetic acid, butyric acid, 
amyl alcohol, pyridine, phenol (5% aqueous solution), resorein (5% aqueous 
solution), hydroquinone (10% aqueous solution), and nitric acid (3N, 6N). 
(3) Twenty four hours’ steeping in an aqueous solution of phenol (5%), 
resorein (5%), acetic acid (1%), and sublimate (0.5%) is fatal to both desic- 
cated and air-dried Oryza grains, whereas the corresponding absolute alcoholic 
or ether solutions are much less injurious. The case with acetic acid, per- 
haps, is of interest. The fixing reagents for plant tissues commonly used in 
the histological and cytological practices, such as chromo-acetic mixture, 
Carnoy’s and Flemming’s fluids, contain a certain amount of acetic acid. An 
