» 146 ISABURO NAGAI: 
plumule. Yoxor (1898) and AKEMINE (1913) show, however, that the 
development of the radiele precedes, if the grains are allowed to germinate 
under a scarcity of available moisture. According to Yoxot, the plumule alone 
develops two or three |days before the radicle develops, if the grains are allowed 
to germinate in a sufficient supply of water; but when the quantity of water 
contained in the medium (sand bed) is seanty (15% —7.5%), the radicle 
develops before the plumule appears. 
Fifty air-dried hulled grains (“Shin-Riki”) are devided into four lots. 
Two lots are allowed to germinate in free access of air, and the other two 
lots are allowed to germinate under anaerobic conditions. One of the two lots 
in each is steeped in water (10mm. deep), and the other is placed on the 
moist filter paper. The grains are put in a vial which is placed in a glass 
cylinder of a capacity of ca. 300c.c. with a tight fitting glass stopper. 30 
e.c. of oxygen absorbent (1 gr. pyrogallol+5 gr. KOH-+30 c.c. H,O), freshly 
prepared, are poured into the cylinder, which is immediately closed with 
the stopper and subsequently sealed up with melting paraffine. As control, 
30 c.c. of distilled water instead of the oxygen absorbent is given and sealed 
up likewise. They are kept in the thermostat at 24C°. After 20 hours, 
germination had not yet taken place; at the end of 41 hours, the grains 
both in water and on the filter paper, in the ordinary air, germinated. In 
the oxygen free air, however, the grains on the filter paper germinated, but 
those in water still remained ungerminated. No growth of root hairs is 
found on the rootlets grown in water, but on those grown on the filter paper, 
they are abundantly formed (cf. Kıees 1884 p. 570-571). At the end of 
five days, the chambers are opened, and the measurement of the length of 
the shoots and roots is made. The result is as follows (Table IX) :— 
