154 ISABURO NAGAT: 
Percentage of Germination 
Seed Condition ae & 
After liquid After high Contact 
air temp. 
Oryza sativa * Araki” hulled, desiccated: 100.0 _ 100 
er: » “ Aka-gome” unhulled, desiccated 88.1 94.1 100 
7 - 3 oe hulled, desiccated 100.0 94.3 100 
A u ATakın unhulled, air-dried — 0 — 
e i “ Chiba-nishiki ” hulled, desiccated — 97.1 = 
As is shown above, the grain of Oryza is much more resistant than 
that of Zea to extremes of temperature, especially to high temperature, at 
least in the materials used. 
VI. Summary. 
In the seed covering of Oryza sativa and of Zea Mays selective per- 
meability is observed. The seat of the selective-permeable septum in the 
Oryza grain is most probably confined to the cutinized inner wall of the 
inner integument which lies directly above the aleurone layer in the fully 
matured grain. 
The germinative power of the desiccated hulled grain of Oryza is slightly 
affected by twenty-four hours’ steeping in 6N sulphuric acid, chloroform, 
acetone, ethyl ether, commercial absolute ethyl alcohol, pierie acid (aqueous 
solution), and the ethyl alcoholic (commercial absolute) solution of thymol, 
naphthalene and «-naphthol, whereas the air-dried grains are killed by 
similar treatment. Likewise in the case with Zea, 5N sulphuric acid, 
hydrochloric acid (twenty one hours), commercial absolute ethyl alcohol, and 
the ethyl alcoholic (commercial absolute) solution of naphthalene, resorcin, 
o-naphthol, and a-naphthylamine destroy the vitality of air-dried grains, but 
not the desiccated ones. 
The vitality of the desiccated grains of Oryza (hulled) and Zea is lost by 
twenty-four hours’ steeping in formaldehyde, formic acid, commercial absolute 
methyl alcohol, methyl ether, acetaldehyde, glacial acetic acid, butyric acid, 
amyl aleohcl, pyridine, and the aqueots solution of chloralhydrate, resorein 
hydroquinone and twenty one hovis’ steeping in nitric acid (3.N, 6 N). 
