344 BUHACHIR® TASAKI AND USHIO TANAKA. 
By the hydrolysis with dilute hydrochloric acid, robitin decomposes into 
a certain yet unknown substance and sugars which are proved by the reduc- 
tion of FEHLING’s solution and osazone formation. 
Robitin solution Reduction 
Hydrolysed (105) much 
» 5%) „ 
Not hydrolysed (1%) negative 
» (3%) » 
The decomposition product is heated in water vapour for 1 hour with 
phenylhydrazin hydrochloride and sodium acetate, a large quantity of osazone 
is formed as bright yellowish crystals and we assumed them to be a mixture 
of glucosazone and ramnosazone by their form. The quantity of sugars was 
estimated by Pavy-KumAGAwA-Surö’s method and amounted to 19.23%. 
Furthermore, glucoside robitin is decomposed by emulsin. 
29% Robitin solution 1% Emulsin solution a 
I 5.0 com. 1.5 cem. (not heated) positive 
I 5 > be 
III > 1.5 ¢cm. (heated) negative 
IV <i + en n 
A » Water 1.5cem. + 
VI Water 5.0 ccm. 1% Emulsin sol. 1.5 cem. (not heated) hr 
VII = es + x (heated) >» 
Robitin contains from 2 to 3% ash, attaining sometimes to 5%. By 
ignition, we recognized that the ash consists chiefly of phosphor, sulphuric 
acid, magnessium, calcium, a little quantity of potassium and sodium and trace 
of iron and chlorine. 
As in the post mortem examination we always found incomplete coagula- 
tion of blood, we made the following test to determine, whether this is caused 
by the hemolytic action of robitin or by suffocation. 
