ON THE TOXIC CONSTITUENTS IN THE BARK OF ROBINIA PSEUDACACIA L. 345 
Dilution Volume of blood taken 1% robitin solution Result 
1 1/2 2 ccm. 1cem. negative 
I 1/4 = ” 
II 1/8 3 „ 
INY 1/16 = ” 
Vi 1/32 + > 
VI 1/64 +, ” 
VII 1/128 r > 
VIII 1/1 Ar physiol. salt sol. 1 cem. 
IV. THe QUANTITY OF RoBITIN IN THE BARK. 
While 3grams of robitin is obtained from 100 grams of the air-dried 
bark of Robinia pseudacacia L., its fresh bark gives 1gram. The outer 
rough portion of the bark contains only one thirtieth of the inner. 
Symptomatology. 
I. Gumea Pic, 
by intravenous injection of robitin into Guinea pigs, the following symp- 
toms are observed: fall of body temperature (Figs. 1, 2), shivering, dyspnoea, 
excretion of feces and paralysis of hind part of body. Frequently it shows 
lacrymal exudation, masticating movement, colic-like symptoms and spasmodic 
cough accompanied with vomiting and sometimes hemorrhagic diarrhoea. The 
Guinea pig dies after a dosis of 0.007 to 0.01 gram per 100 grams body 
weight (as lcem. solution) by intravenous injection, showing stop of respira- 
tion after 15 minutes to 3 hours. 
By the hypodermic injection, almost the same symptoms are observed as 
by the intravenous, but they appear more slowly. 
