356 ON THE TOXIC CONSTITUENTS IN THE BARK OF ROBINIA PSEUDACACIA L. 
Time iY R. Ps Symptoms 
55/ | 38.8 | 106 56 | Defzecation (0.5 litre, muddy), tenesmus, 
2° | — —_ — 4 (1 litre, muddy), 
47) — 110 — Body leaning on the side wall. 
107; — _ _ Diarrhea (1 litre). 
15 | 38.4 | 94 64 Watery diarrhaa. 
25/| — — — Diarrhea. 
26 | — 80- — Watery diarrhcea, breathing becomes quiet, thenceforth symp- 
toms gradually disappeared, but to to-morrow morning 
sometimes diarrhea, . 
Summary. 
1. A new toxic glucoside, named ‘ Robitin’, was isolated from the bark 
of Robinia pseudacacia L. Its quantity in the fresh bark amounts to 1%. 
2. The intoxication by robitin is recognized in cattle, horse, rabbit and 
Guinea-pig. The chief symptoms are dyspnoea, increase of secretions and 
excretions and paralysis of the hind part of the body. In the post mortem 
examination, we observe emphysema and hemorrhagia of lungs and incomplete 
coagulation of the blood. | 
3. Robitin causes toxic reaction by doses of 0.0015 grams in horses, 
0.02 grams in cattle, 0.07 grams in Guinea-pigs and 0.5 grams in rabbits 
per 1 kilo. body weight. 
4, The reactions observed by the injection of robitin into horses agree 
exactly with the symptoms in the experiment with the fresh bark. 
We have to express to Assistant Prof. T .SuimAMuURA our great indebted- 
ness for his advice so kindly given during the progress of the work. 
