444 BULLETIN OF THE BUSSEY INSTITUTION. 
consists with the view, not infrequently enunciated, that appre- 
ciable quantities of starch are stored in the winter in the roots 
of trees. 
As was the case in the experiment described on page 440, so 
here, the long continued boiling appeared to have produced rever- 
sion (or destruction?) of a part of the dextrose that had been 
formed at first. On mixing what remained of the two solu- 
tions from the hydrolysis with 3% hydrochloric acid, they were 
evaporated nearly to dryness and the residue was treated with 
strong alcohol. The matter insoluble in alcohol gave quasi 
(a) D = 84°.69, and the matter soluble in alcohol gave quasi 
(ny Dies 39-41: 
The residual wood left after hydrolyzing with hydrochloric acid 
of 3% was soaked in 80 ¢.c. of strong hydrochloric acid of 35% 
during 24 hours in the cold, and the mixture was poured into 
enough boiling water to dilute the acid to a strength of 3%. 
The diluted mixture was boiled during three hours and then 
filtered. One half the filtrate, neutralized with sodium hydrox- 
ide, evaporated, and decolorized with bone-black gave quasi 
(a) D = 76°.78 and ‘‘dextrose” to the amount of 7.33% of 
the dry material operated upon, or to 5.64% of the dry wood that 
had been leached with ammonia. 
The other half of the acid filtrate, after having been boiled 
three hours longer, gave anomalous results, as to rotatory power, 
while Fehling’s liquor showed only 2.94% of ‘* dextrose” calcu- 
on May Ist. A hardly visible reaction with iodine was got, along the medul- 
lary rays, on testing the outer and inner wood of a maple tree felled on 
May 380th. On examining wood and bark from the trunk of a sugar maple 
tree, felled in October, he got no reaction for starch in the bark; and the 
outer wood gave the starch reaction only in the medullary rays, excepting a 
few cases where scattering starch grains were detected in the wood fibre, 
usually not far from the medullary rays. Some starch grains were observed 
in the medullary rays of the inner wood also, but they were few in number, 
a. e. they were still less numerous than those observed in the outer wood of 
the same tree. 
Pith from a stem of an elder bush (Sambucus) cut in July gave no reaction 
with iodine. Wood cut from the stem of a red maple tree in June gave a 
decided reaction along the medullary rays, while wood cut on the same day 
from a gray birch tree showed only a slight coloration with iodine; indeed, 
there were so few of the blue colored granules lying in the medullary rays 
that they could readily be counted. 
