BULLETIN OF THE BUSSEY INSTITUTION. 445 
lated on the dry wood that had been leached with ammonia. To 
all appearance much of the sugar produced in the first hydrolysis 
had been destroyed by the second boiling. 
The residual wood, from the foregoing hydrolysis with strong 
hydrochloric acid, was treated with 10 c.c. of concentrated sul- 
phurie acid (of 92.5% H,SO,) and left to stand in the cold 
during 24 hours. It was then poured into enough boiling water 
to bring the mixture to a strength of 3% H,SO,, and the whole 
was boiled for three hours. The filtered liquid was divided into 
two parts. One part was neutralized with calcium carbonate and 
evaporated. It did not need to be decolorized. The solution 
gave quasi(a) D — 73°.48 and ‘* sugar” enough to amount to 
32.12% of dextrose in the dry matter operated upon, or to 22.91% 
in the dry wood that had been leached with ammonia. 
The rest of the neutralized solution was evaporated to dryness 
and the residue was treated with, hot, strong alcohol. The light 
brown, sticky mass insoluble in alcohol did not need-to be decol- 
orized when taken up with water. It gave quasi(a) D = 79°.34, 
and Fehling’s liquor indicated 19.29% of sugar calculated as dex- 
trose on the dry wood that had been leached with ammonia. 
The matter soluble in alcohol, on being dissolved in water, 
after the expulsion of the alcohol, gave quasi(a) D = 54°.60, 
and Fehling’s liquor showed dextrose enough to amount to 18.93% 
of the dry wood that had been leached with ammonia, 
The second portion of the acid filtrate from the hydrolysis with 
3% sulphuric acid (after the action of strong sulphuric acid) was 
boiled three hours longer, after it had been removed from the 
wood. It was then neutralized with calcium carbonate and evap- 
orated. The solution did not need to be decolorized. It gave 
quasi(a) D = 68°.58, and Fehling’s liquor showed 33.16% of 
‘¢ dextrose” calculated on the dry substance operated upon. On 
being again evaporated, it was treated with hot, strong alcohol. 
The matter insoluble in alcohol on being dissolved in water 
eave quasi(a) D= 58°.33, and Fehling’s liquor showed 22.92% 
of ‘‘dextrose.” The matter soluble in alcohol gave quasi 
(a) D = 66°.30, and Fehling’s liquor showed 9.45% of dextrose. 
It will be noticed that, as indicated by Fehling’s test, the total 
amount of sugar obtained from the three prime hydrolyses of the 
maple root was by no means inconsiderable. After the first 
