BULLETIN OF THE BUSSEY INSTITUTION. 461 
with strong sulphuric acid, and noting the reducing and quasi- 
rotatory power of the products. Thus, 10 grm. of dextrose 
anhydride were mixed with 14 c.c. of sulphuric acid of 90% 
H,.SO,, and the mixture was allowed. to stand for 24 hours. No 
immediate coloration occurred, but gradually the mixture turned 
brown, and at the end of 24 hours it had become black. The 
mixture was poured into enough boiling water to reduce the acid 
to a strength of 3.5% H,SO,, and the boiling was continued for 
three hours. The resulting solution was almost colorless; it 
exhibited only a slight brown or yellowish tint which did not in- 
crease in the course of the subsequent processes of neutralization 
and evaporation. -There was no need of decolorizing the solutions 
before testing their rotatory power. 
One part of the product of the hydrolysis was neutralized with 
calcium carbonate and evaporated to a thin syrup, which gave 
quasi (a) D = 78°.61, and reduced enough Fehling’s liquor to 
indicate the presence of 69.76% of dextrose, calculated, of 
course, on the quantity of dry dextrose that was treated with 
sulphuric acid. A portion of this syrup was evaporated to a 
thick syrup, which was treated with successive portions of cold, 
strong alcohol until nothing more was dissolved. There was 
left a slimy, sticky mass similar in appearance to those ob- 
tained by similar treatment. from wood and from cotton. It 
gave quasi(a) D = 73°.33. 
Another part of the acid liquor which resulted from the original 
hydrolysis was neutralized with lead carbonate, and the filtrate 
therefrom was treated with sulphuretted hydrogen; and these 
processes were repeated a second time for the sake of certainty. 
After boiling off the excess of sulphuretted hydrogen, the clear 
solution gave quasi(a) D = 66°.45, and Fehling’s liquor showed 
83.39% of ‘* dextrose.” The solution evaporated to a syrup, 
and treated with strong alcohol, in which much of it dissolved, 
left an insoluble residue which gave quasi(a) D = 88°. 
Having in view the well-known difficult solubility of anhydrous 
dextrose in strong alcohol, one or two experiments were made for 
the sake of contrasting dextrose precipitated by alcohol with the 
‘¢matter insoluble in alcohol” mentioned on foregoing pages. 
To this end a quantity of pure dextrose anhydride (from Merck 
