THE AMOUNT AND THE DISTRIBUTION OF CREATI- 
NINE AND CREATINE IN NORMAL HUMAN BLOOD. 
By ANDREW HUNTER anp WALTER R. CAMPBELL. 
(From the Department of Pathological Chemistry, University of Toronto.) 
(Received for publication, November 17, 1917.) 
Of the non-protein constituents of blood, some, such as potas- 
sium,! phosphoric acid,!'? lecithin,? free cholesterol? 4:5 and 
amino-acids,*» 7» are localized to a greater or smaller extent 
within the corpuscles; some, such as sodium,! calcium, chlorine,! 
cholesterol esters,> glucose,? and adrenalin,!® are more abundant 
in, or even entirely confined to the plasma; some, finally, with 
urea’: " as their most conspicuous known representative, main- 
tain a practically equal concentration in both. There are few 
important constituents of which the relative concentration in 
1 Abderhalden, E., Lehrbuch der Physiologischen Chemie, Berlin and 
Vienna, 1906, 591-593. ¢ 
2 Porte, A., Compt. rend. Soc. biol., 1914, Ixxvii, 467. 
3 Bloor, W. R., J. Biol. Chem., 1916, xxv, 577. 
4 Hepner, E., Arch. ges. Physiol., 1898-99, Ixxiii, 595. 
6 Bloor, W. R., and Knudson, A., J. Biol. Ghem., 1917, xxix, 7. 
6 Bang, I., Biochem. Z., 1915, Ixxii, 104; 1916, Ixxiv, 294. 
Book s..0,, 0.201. Chem, 1917, xxix,1191. 
8 Wilson, D. W., and Adolph, E. F., J. Biol. Chem., 1917, xxix, 405. 
®Macleod, J. J. R., Diabetes; Its Pathological Physiology, London, 
1913, 31. The distribution of glucose in the blood is, however, still the 
subject of debate. Quite recently Gradwohl and Blaivas (J. Lab. and 
Clin. Med., 1916-17, ii, 416), using the method of Lewis and Benedict, 
‘found the sugar content of the corpuscles in man to be nearly always 
the same as that of the plasma. 
10 Stewart, G. N., and Rogoff, J. M., Proc. Soc. Exp. Biol. and Med., 
1916-17, xiv, 79. 
11 Marshall, E. K., and Davis, D. M., J. Biol. Chem., 1914, xviii, 53. 
Karr, W. G., and Lewis, H. B., J. Am. Chem. Soc., 1916, xxxviii, 1615. 
In fish blood urea is chiefly localized in the corpuscles; see Wilson and 
Adolph.é 
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