STUDIES OF ACID PRODUCTION. 
II. THE MINERAL LOSS DURING ACIDOSIS.* 
By MARGARET SAWYER, L. BAUMANN, anv F. STEVENS. 
(From the Departments of Home Economics and Internal Medicine, State 
University of Iowa, Iowa City.) 
(Received for publication, November 19, 1917.) 
The effect of acid administration and increased acid produc- 
tion on the excretion of certain minerals and nitrogen has been 
repeatedly studied. Recent discussions of the literature and 
bibliographies will be found in the publications of Forbes and 
Keith,’ Givens and Mendel,’ and Stehle. 
A survey of the literature failed to show a record of a com- 
plete experiment on the normal growing human organism. As 
children.are peculiarly prone to acidosis it appeared desirable to 
supply the missing data. It was with this object in view that the 
following experiments were undertaken. 
Methods. 
The observations were made in a metabolism ward on two 
male children aged 5 and 8 years, and weighing 22.7 and 23 
kilos, respectively. The boys were under the supervision of a 
special nurse.. The food was prepared by a trained dietitian. 
Each period of observation lasted 3 days. 
Each child consumed 436 gm. of egg white, 74 gm. of egg yolk, 414 gm. 
of milk, 64 gm. of pure sucrose, 60 gm. of pure lactose, 2 gm. of pure so- 
dium chloride, and 3 gm. of agar, daily during the normal period. This 
amount of food contained 324 protein, 385 fat, 593 carbohydrate calories, 
and 12.6 gm. of nitrogen. The energy intake was equivalent to 56 cal- 
ories per kilo. During the period of high fat feeding the protein calories 
remained the same, but the carbohydrate calories were reduced to 85, 
* The expenses of the research were met in part by a grant from the 
research fund of the Graduate College of the State University of Iowa. 
1 Forbes, E. B., and Keith, M. H., Ohio Agric. Exp. Station Technical 
Bull. 5, 1914. 
2 Givens, M. H., and Mendel, L. B., J. Biol. Chem., 1917, xxxi, 421. 
2 Stehle, R. L., J. Biol. Chem., 1917, xxxi, 461. 
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