100 Plasma Chlorides and Chloride Excretion 
Experiment 11 A.—No food or water since previous day. 
Feb. 12, 3.00 p.m. Catheterized. Given 100 cc. of distilled water by 
stomach tube. 3.45 p.m. Bled 30 cc. 4.30 p.m. Catheterized. 30 cc. 
of urine (90 minutes). N, 6.2; D, 0.6; E, 1.2; X, 0.0133; blood urea N, 14. 
Experiment 12.—Low salt diet. Same diet and distilled water as in 
Experiments 9, 10, and 11 (Feb. 13 to 20). 
Experiment 12 A.—No food or water since previous day. 
Feb. 21. 3.12 p.m. Catheterized. Given 100 cc. of distilled water by 
stomach tube. 3.52 p.m. Bled 25 cc. 4.37 p.m. Catheterized. 37 ce. 
of urine (85 minutes). N, 6.2; D, 0.36; E, 0.6; X, 0.0133. 
Experiment 13.—High salt diet. Dog placed 13 days (Feb. 28 eo Mar. - 
12) on milk, 1 quart, and one dog biscuit daily. Given 150 cc. of 5 per 
cent sodium chloride solution daily by stomach tube. Water ad libitum. 
Experiment 13 A.—No food, water, or salt since previous day. 
Mar. 18, 3.25 p.m. Catheterized. Given 100 ce. of distilled water by 
stomach tube. 4.05 p.m. Bled 30 cc. 4.40 p.m. Catheterized. 13 ce. 
of urine (75 minutes). N, 6.35; D, 1.5; E, 5.8; X, 0.0134, blood urea N, 14. 
Experiment 14.—High salt diet. Same diet and sodium chloride as in 
Experiment 13 (Mar. 14 to 19). Same diet, no sodium chloride, Mar. 20. 
Experiment 14 _A.—No food or water since previous day. 
Mar. 21, 2.00 p.m. Catheterized. Given 100 cc. of distilled water by 
stomach tube. 2.85 p.m. Bled 30 ce. 3.05 p.m. Catheterized. 32 cc. 
of urine (65 minutes). N, 6.2; D, 0.5; E, 0.7; blood urea N, 13. 
CONCLUSIONS. 
1. The level of plasma sodium chloride 24 hours after feeding 
varied in three dogs from 5.9 to 6.3 gm. per liter, but was quite 
constant for any one animal. 
2. By administering large amounts of sodium chloride in solu- 
tion by stomach tube, the level of the plasma chlorides can be 
raised in the dog within 14 hours, 1 gm. per liter. The return 
to the previous level occurs within from 24 to 48 hours. 
3. It is not possible to raise the level of the plasma chlorides 
’ other than in this transient manner by a prolonged régime on a 
high chloride diet, or to lower the plasma chlorides below the 
normal level even by a prolonged régime on a very low chloride 
diet. 
4. In certain nephritic animals with impaired chloride excretion, 
whatever may be the hypothetical chloride threshold, a given in- 
crement in the plasma chloride concentration excites less incre- 
ment in the rate of chloride excretion than it would in a normal 
animal. 
