EK. V. McCollum and N. Simmonds 89 
structive action of heat. The alcohol was distilled off, the ex- 
tract taken up in water, and made up to 550 cc. It was dis- 
tinctly acid to litmus. It was neutralized to. phenolphthalein 
with a sodium hydroxide solution and a sufficient excess added 
to make it alkaline up to 0.28 per cent NaOH. At this degree of 
alkalinity the solution was heated for 1 hour under a reflux, 
then cooled, then almost neutralized with hydrochloric acid, and 
acidified by the addition of 12 per cent acetic acid which made 
it strongly acid. It was evaporated on dextrin in large granite 
pans and dried at 70—-80° for 5 hours. 
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