ADAM McCollum and N. Simmonds 87 
Chart 12. Lots 995 A, 996 B, and 972 A. 
Lot 995 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 plus a preparation of water-sol- 
uble B treated 4 hours with nitrous acid. An extract equivalent to 3 per 
cent of wheat embryo was fed. 
Period 3.—Ration: Same as Period 2, but with the water- soluble B 
preparation treated with nitrous acid increased to the equivalent of 10 
per cent of wheat embryo. 
It is possible that treatment with nitrous acid tends to de- 
stroy the dietary factor B to a slight extent, but it does so very 
slowly, if at all. The resistance of this substance to nitrous 
acid is a strong indication that it is neither a primary nor a 
secondary amine. 
Lot 995 B. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1, but with a preparation contain- 
ing the water-soluble B equivalent to 3 per cent of wheat embryo in the 
food mixture. This preparation was evaporated with hydrochloric acid 
as described below. 
Period 3.—Ration: Same as Period 2 with the water-soluble B prepa- 
ration increased to the equivalent to 10 per cent of wheat embryo. 
It is not clear from this experiment whether or not the treatment 
with hydrochloric ‘acid destroyed a part of the dietary factor B. 
The fact that there was no growth in Period 3 would seem to 
indicate that the substance is slowly inactivated. This phase 
of the subject will receive further study. Relatively great 
stability toward hydrochloric acid is clearly shown. 
