86 Water-Soluble B 
14 hours. 25 per cent of ether-soluble material was thus re- 
moved, leaving 360 gm. of residue. 
112.9 gm. of the above ether-free residue were extracted with 
acetone in three consecutive 6 hour periods, changing the flask 
at the end of each period. The first two 6 hour periods gave 
4.572 gm. of extract, the last 6 hour period 2.26 gm. They were 
taken up in alcohol and evaporated on dextrin at 40°. The final 
weight of extracts on dextrin was 34.4 gm. This extract (ace- 
tone-soluble material) was fed in the ration of Lot 960 B, Period 
2. The residue after extraction (acetone-insoluble material) was 
fed in the ration of Lot 961 B, Period 2. 
Preparation of Extract and Residue Employed with Lots 961 A 
and 962 A_ Respectivelyn—l22 gm. of ether-extracted kidney 
residue obtained as previously described were extracted with 
benzene in three consecutive 6 hour periods changing the flask 
with the extract at the end of each period. 4.10 gm. of extract 
were obtained in the first two 6 hour periods and 0.051 gm. in. 
the last. Both fractions were united, and were then evaporated 
on dextrin yielding 28.15 gm. The benzene-soluble matter was 
fed to Lot 961 A, Period 2; the benzene-insoluble residue was 
fed to Lot 962 A, Period 2. 
The Stability of the Water-Soluble B toward Nitrous Acid, Hydro- 
chloric Acid, and Dilute Sodium Hydroxide. 
The studies of Williams and his coworkers (3) and of Sullivan 
and Voegtlin (3) have indicated that the substance which cures 
polyneuritis in fowls is rapidly destroyed by even moderately 
dilute alkalies, but is relatively stable toward mineral acids. We 
have examined preparations of the water-soluble B prepared from 
wheat embryo by extraction with alcohol after removal of the 
fats by means of ether, for their power to induce growth in rats. 
The preparations, previous to feeding, were treated with one of 
the following reagents: (a) nitrous acid, (6b) hydrochloric acid, 
(c) sodium hydroxide. Our results with alkalies are in agree- 
ment with those of Williams and Sullivan and Voegtlin. 
