E. V. McCollum and N. Simmonds 79 
tract of 30 gm. of ether-extracted heated beans did not in Lot 
973 B suffice to furnish enough of the factor B to induce growth, 
but growth at a moderate rate took place when the equivalent 
of 60 gm. of beans per 100 gm. of ration was included. Chart 
10, Lot 933, shows, however, that even 10 per cent of cooked 
navy beans suffices to induce normal growth during 7 weeks. 
There may be slight destruction of the water-soluble B during 
cooking of beans but destruction proceeds very slowly, if at all. 
The explanation of the failure of the extracts of cooked beans 
to support growth as well as extracts of similar amounts of raw 
beans, lies in the failure of alcohol to extract the water-soluble B 
150{| LOT 3000 & LOT 9 A 
110 ve 
Cee | 
very. efficiently from cooked beans. This is apparently due to 
change in the physical properties of the bean. 
Lot 973 B. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1, but with the benzene extract of 
the alcohol-soluble part of 30 gm. of ether-extracted cooked navy beans 
per 100 gm. of ration; 7.e., the alcohol-soluble, benzene-soluble portion 
was fed. 
Period 3.—Ration: Same as in Period 2, but with the eotract increased 
to the equivalent of 60 per cent of beans. 
This experiment might seem to suggest that there is progressive 
destruction of the factor B on cooking the beans (compare Chart 
1, Lot 953 A, Period 3). The benzene extract of the alcoholic 
extract of 30 gm. of raw beans, prepared in the same manner as 
the extract in this experiment, suffices for the promotion of 
growth. Chart 10, Lots 932 and 933, however, makes it evident 
