78 Water-Soluble B 
Preparation of the Extracts Employed with Chart 7, Lot 1,000 A 
and Chart 8, Lot 1,000 B.* 
extraction with.ether for 18 hours were extracted with hot aleo- — 
hol for 18 hours in 3 consecutive 6 hour periods, changing the 
flasks containing the extracts at the end of each period. ‘The 
first 12 hours’ extraction yielded 45 gm. of extractives, the last 
6 hours’ 10.2 gm. The extracts were evaporated on dextrin, 
united, and made up to 200 gm. with dextrin. This extract was 
used in the ration of Chart 7, Lot 1,000 A, equivalent to 10 per 
cent of beans, and in Chart 8, Lot 1,000 B, seen en to 15 per 
cent of beans. 
Chart 8. Lots 1,000 B, 978 A, and 973 B. 
Lot 1,000 B. Period 1.—Ration: Purified food mixture plus 5 per cent 
of butter fat. 
Period 2.—Ration: Same as Period 1, but with the addition of the hot 
alcoholic extract of 15 gm. of ether-extracted raw navy beans per 100 gm. 
of ration. 
The animals in this lot which received the alcoholic extract of 
15 per cent of raw beans were but little better off than those in 
Chart 7, Lot 1,000 A, which got their supply of the factor B from 
the alcoholic extract of 10 per cent of beans. Incomplete extrac- 
tion appears to be unavoidable when beans are used. 
Lot 973 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1, but with the residue of the ben- 
zene extract of the alcoholic extract of 30 per cent of ether-extracted, 
cooked navy beans per 100 gm. of ration; 7.e., the alcohol-soluble, benzene- 
insoluble portion was fed. 
At first thought this curve might give the impression that in 
the process of heating with water a progressive destruction of the 
water-soluble B takes place. 25 per cent of navy beans heated 
for 1; hours at 15 pounds’ pressure still induces normal growth 
when combined with a suitable mixture of purified foodstuffs (5) 
plus 5 per cent of butter fat. In Chart 1, Lot 953 A, Period 3, 
it was shown that the benzene extract of the alcoholic extract 
of 30 per cent of ether-extracted raw navy beans sufficed to 
induce good growth. The benzene extract of the alcoholic ex- 
