EK. V. MeCollum and N. Simmonds cies 
Period 2.—Ration: Same as Period 1 but with the addition of 3 per cent 
of wheat germ which had been extracted with ether and afterwards with 
hot chloroform for 18 hours. 
This experiment shows that neither ether nor hot chloroform 
takes out the water-soluble B from wheat embryo. 
Lot 1,000 A. Period 1.—Ration: Purified food mixture plus 5 per cent 
of butter fat. 
Period 2.—Ration: Same as Period 1 but with the alcoholic extract of 
ether-extracted raw beans equivalent to 10 per cent of beans. 
It has been shown previously that it is very difficult, if at all 
possible, to extract completely the water-soluble B from beans 
200 ‘a 955 A aie 5A LOT 1000 A} 
aaa 
aoe ee et 
Ze 
ee AE 
by means of hot alcohol (Chart 3, Lot 951 A, Period 3). This is 
doubtless due to the great difficulty of grinding beans to an 
impalpable powder. 
Preparation of the Extract and Residue Employed with Chart 7, 
Lots 955 A and 956 A.—589 gm. of wheat embryo previously 
extracted with ether for 18 hours were extracted with chloro- 
form in three consecutive 6 hour periods. 0.838 gm. was 
extracted in the first two 6 hour periods and 0.218 gm. in the last. 
The extracts were taken up in chloroform, evaporated on dex- 
trin, and made up to 58.9 gm. with dried dextrin. All evaporat- 
ing and drying operations were carried out with the least possible 
exposure to heat. The extract (chloroform-soluble material) was 
used in the ration of Lot 955 A. The chloroform-extracted 
residue of wheat embryo was employed in the ration of Lot 956 A. 
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