76 Water-Soluble B 
Lot 789 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 plus 3 per cent of the ether- and 
benzene-extracted residue of wheat embryo. 
This experiment shows that hot benzene does not remove the 
water-soluble B from ether-extracted wheat germ directly. This 
is in agreement with the results obtained with beans (Chart 1, 
Lot 953 A, Period 2, and Chart 2, Lot 994 A, Period 2). 
Lot 791 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 plus the benzene extract of 14.28 
per cent of ether-extracted wheat embryo. 
Period 3.—Ration: Same as Period 2 plus 3 per cent of the ether- and 
benzene-extracted residue of wheat embryo. 
Two rats had polyneuritis in Period 2. In Period 3 the ration 
caused prompt recovery. This experiment shows that benzene 
does not remove the water-soluble B directly from wheat em- 
bryo. The residue fed in Period 3 still contained the physio- 
logically active substance. 
Preparation of Extract and Residue Employed with Chart 6, Lots 
789 A and 791 A.—1,000 gm. of wheat embryo which had been 
extracted 18 hours with alcohol-free ether were extracted with hot 
benzene continuously for 18 hours. Most of the benzene was 
then distilled off and the residue which was readily soluble in a 
little benzene was evaporated in a weighed crystallizing dish and 
dried 7m vacuo 24 hours. The weight of the material dissolved by 
benzene was 3.629 gm. This was again taken up in benzene and 
evaporated on dextrin, and made up to 200 gm. with dried dex- 
trin. This preparation was employed in the ration of Lot 791 A, 
Period 2. 
The benzene-insoluble material was used in the rations of Lot 
789 A, Period 2, and also in Lot 791 A, Period 3. 
Chart 7. Lots 955 A, 956 A, and 1,000 A. 
Lot 955 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 plus the chloroform extract of 
ether-extracted wheat embryo equivalent to 15 per cent of wheat embryo. 
Lot 956 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
