74 Water-Soluble B 
Lot 836 R. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 plus 3 per cent of the residue after 
ether and acetone extraction of wheat embryo. 
This experiment shows that acetone does not take out to any 
appreciable extent the water-soluble B from ether-extracted 
wheat embryo. Studies with pigeons indicate that traces are 
removed by this solvent, for the acetone extract of wheat embryo 
relieves polyneuritic pigeons (4). 
Lot 836 E. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 but with the acetone extract of 
ether-extracted wheat embryo equivalent to 14.28 per cent of wheat 
embryo in the diet. 
This experiment shows that acetone takes out the water- 
soluble B from wheat embryo to a very slight extent. 
Preparation of the Extract and Residue Employed with Chart 4, 
Lots 836 R and 886 E; Chart 6, Lot 8386 R + E.—500 gm. of wheat 
embryo previously extracted with alcohol-free ether for 18 hours 
were .extracted continuously with hot acetone in a Soxhlet appa- 
ratus for 18 hours. The acetone was distilled off, the residue taken 
up with hot water, and evaporated on 300 gm. of dry dextrin. 
After drying, 3.2 gm. were found to have been removed in the ex- 
traction process. The extract was made up. to 400 gm. with 
dextrin. 
Chart 6. Lots 886 R+E, 789 A, and 791 A. 
Lot 886 R+ EH. Period 1.—Ration: Purified food mixture plus 5 per 
cent of butter fat. 
Period 2.—Ration: Same as Period 1 but with the addition of 3 per cent 
of the ether- and acetone-extracted residue plus the acetone extract of 14 
gm. of ether-extracted wheat embryo per 100 gm. of ration. 
This experiment shows that the water-soluble B is not rapidly 
destroyed by prolonged treatment with hot acetone. There was 
no evidence from the appearance or activity that these rats re- 
ceiving both the acetone extract and residue as in 8386 R+ E 
were in better condition than those receiving only the residue, as 
in Chart 5, Lot 886 R. This supports the view that there is 
but a single substance in what we term water-soluble B. 
