fa Water-Soluble B 
Period 2.—Ration: Same as Period 1 plus the acetone-soluble part of 
the alcoholic extract of ether-extracted cooked navy beans equivalent to 
60 gm. of beans. 
This experiment shows that the water-soluble B is slightly 
soluble in acetone but very much less so than in benzene (com- 
pare with Chart 1, Lot 953 A, Period 3, and Chart 5, Lot 1,001 C, 
Period 2). 
Preparation of Extract and Residue Kmployed with Lots 1,001 A, 
1,001 B, and 1,001 C.—935 gm. of cooked navy beans were ex- 
tracted with ether for 18 hours. The residue, freed from ether, 
was then extracted with alcohol for 18 hours in three consecutive 
6 hour periods, changing the flask containing the extract at the 
end of each period. The first 12 hours’ extract evaporated on 
dextrin gave 26.7 gm. of material, the last 6 hours’ gave 7.6 gm. 
The extracts were united and made up to 200 gm. with dextrin. 
100 gm. of the above preparation equivalent to 467-gm. of — 
beans were extracted with freshly redistilled acetone, as outlined 
above. The first 12 hours’ extraction gave 14.7 gm. of extract, 
the last 6 hours’ 0.5 gm. The extracts were united and made 
up with dextrin to 100 gm. This preparation was employed 
with Lot 1,001 B, Period 2. The residue was likewise made up 
to 100 gm. after the extraction. This preparation was em- 
ployed with Chart 5, Lot 1,001 C, Period 2. Both the ex- 
tract and residue were employed in the ration of Lot 1,001 A, 
Period 2. 
Chart 5. Lots 1,001 C, 836 R, and 836 E. 
Lot 1,001 C. Period 1.—Ration: Purified food mixture plus 5 per cent 
of butter fat. 
Period 2.—Ration: Same as Period 1 but with the residue of the ace- 
tone extract of the alcoholic extract of ether-extracted cooked navy beans 
equivalent to 60 per cent of beans; 7.e., the alcohol-soluble, acetone-in- 
soluble part. 
This experiment shows that in time hot acetone separates a 
part of the water-soluble B from the cooked navy beans (see 
Chart 4, Lot 1,001 B), but leaves the larger part of it in the 
residue. Rats grow on both the acetone extract and its residue 
when the plane of intake is sufficiently raised. 
