E. V. MeCollum and N. Simmonds 71 
Chart 4. Lots 951 B, 1,001 A, and 1,001 B. 
Lot 951 B. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1, but with the acetone-soluble part 
of the alcohol-soluble part of ether-extracted raw navy beans, equivalent 
to 30 per cent of beans in the diet. 
Period 3.—Ration: Same as Period 2 but with the addition of the ace- 
tone-insoluble part of the alcohol-soluble part of beans, equivalent to 30 
per cent of beans; 7.e., the equivalent of the entire alcohol-soluble part 
of the beans, after being heated with acetone. 
This experiment shows that even after extraction with alcohol, 
the water-soluble B is but slightly soluble in acetone. This con- 
clusion is further supported by Chart 4, Lot 1,001 B, Period 2, and 
Chart 5, 1,001 C, Period 2. 
3a 
190 ee | das 
150 |. a 
CS ee a 
= ee Ae 
70 a 
our A 
Weeks 
In Chart 1, Lot 952 A, Period 2, it is shown that no growth 
could be secured with the alcohol-soluble, acetone-insoluble por- 
tion of beans equivalent to 30 per cent of beans in the diet. 
This: suggests that there is a slow destruction of the water-soluble 
B during heating with acetone. 
Lot 1,001 A. Period 1.—Ration: Purified food mixture plus 5 per cent 
of nee fat. 
Period 2.—Ration: Same as Period 1 plus the acetone extract of the 
alcoholic extract and*residue of same, of 10 gm. of ether-extracted cooked 
navy beans; 7.e., the equivalent of the alcoholic extract of navy beans 
after being heated with acetone. 
Lot 1,001 B. Period 1.—Ration: Purified food mixture plus 5 per cent 
of butter fat. 
30 
