70 Water-Soluble B 
acetone in three consecutive 6 hour periods changing the flask 
containing the extract each time. On distilling off the acetone, 
3.77 gm. were found to have been removed by extraction in the 
first two 6 hour periods and 0.75 gm. in the last. They were 
taken up in a small amount of ether and evaporated on dextrin 
— (95.5 gm:) giving a final weight of 100 gm. This extract was fed 
in the ration of Lot 954 .B, Period 2. The residue (that which 
was Insoluble in acetone) was later heated and fed in the ration of 
Lot 954 A, Periods 2 and 3. 
Preparation of Extract and Residue Used in Chart 1, Lot 952 A, 
Period 2, Chari 3, Lot 951 A, Period 2, and Chart 4, Lot 951 B, 
Period 2.—1,000 gm. of finely ground raw navy beans were ex- 
tracted with alcohol-free ether for 25 hours. Upon evaporating 
off the ether, 13.30 gm. of material were found to have been 
removed by ie extraction. 
The residue from the above extraction was fap from ether at 
room temperature and then extracted with 95 per cent alcohol in 
three consecutive 6 hour periods, using fresh or recovered sol- 
vent each time in order to minimize the possible destructive ac- 
tion of heat on the extract. 25.5 gm. of material were left as the 
extract of the first two 6 hour periods after distilling off the alco- 
hol and 7.85 gm. from the last 6 hour period. These extracts 
were taken up in a little water and alcohol and then evaporated 
on dextrin and dried at 90—95° for a few hours. The dry weight 
was 128.35 gm. The residue from the extraction was freed from 
alcohol by heating at 90° for 3 hours. Later part of this residue 
was soaked in water and heated in the autoclave at 15 pounds’ 
pressure for 40 minutes. This preparation (alcohol-insoluble) was 
fed to Lot 951 A,,Chart 3, Period 2. 
The 128.35 gm. of dried dextrin residue carrying the aleoholic 
extract from 1,000 gm. of raw navy beans was next extracted with 
acetone ina Seal Soxhlet apparatus, again in three consecutive 6 
hour periods. After removing the acetone with minimum heat 
exposure, 7.96 gm. of extract were left from the first two 6 hour 
periods and 0.81 gm. from the last 6 hour period. ‘They were 
taken up in alcohol, evaporated on dextrin. and made up to 100 
gem. This mixture was fed to Lot 951 B, Chart 4, Period 2. The 
residue from the acetone extraction was freed from acetone and ~ 
made up to 125 gm. with dextrin. It was employed in the 
ration of Lot 952 A, Chart 1, Period 2. 
