E. V. McCollum and N. Simmonds 69 
Lot 954 B. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 with an acetone extract of ether- 
extracted raw navy beans equivalent to 30 per cent of beans in the diet. 
Acetone does not readily extract the water-soluble B from raw 
beans (see Chart 4, Lot 1,001 B, Period 2). The acetone-soluble 
part of the bean does not induce appreciable growth, but the ace- 
tone-insoluble cooked residue does (Chart 3, Lot 954 A, Period 3). 
Lot 951 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 but with 25 per cent of the residue 
of raw navy beans after extraction with ether, then with alcohol. 
oye A pepe} forme amet 
Sect 
2 a 
ect || def | ed 
Period 3.—Ration: Same as Period 2, but with the bean residue moist- 
ened with distilled water and heated in an autoclave 40 minutes at 15 
pounds’ pressure. 
( This experiment shows that the method of alcoholic extraction 
employed did not remove all of the water-soluble B from the 
beans. ‘The residue was still potent in promoting growth. 
Preparation of Extract and Residue Used with Lots 954 A; 
Period 2, and 954B, Period 2.—1,000 gm. of finely ground raw 
navy beans were extracted continuously with alcohol- free ether 
for 241 hours: 13.19 gm. of material were.extracted. The residue 
was freed from ether at room temperature and extracted with 
! 
