66 Water-Soluble B 
hour periods using fresh solvent each time to reduce the amount 
of exposure to heat. 1.635 gm. of material were extracted in the 
first two 6 hour periods and 0.222 gm. was extracted in the last. 
They were made up to 100 gm. with dried dextrin. The residue 
was freed from benzene and later heated in the autoclave. The 
benzene-soluble part was employed in the ration of Lot 953 A, 
Period 2. The benzene-insoluble part was used with Lot 952 B, 
Period 2, and, after heating, in Period 3. For the alcohol-soluble, 
acetone-insoluble fraction, employed in Chart 1, Lot 952 A, 
Period 2, see under 951 A, Chart 3. , 
Preparation of the Extract and Residue Employed with Lots 
953 A, Period 3, and 953 B, Period 2.—1,000 gm. of finely ground 
raw navy beans were extracted for 25 hours with alcohol-free 
ether. 13.33 gm. of material were thus removed. The extracted 
residue freed from ether was then extracted with 95 per cent 
alcohol in three successive 6 hour periods. After distilling off the 
alcohol 30.45 gm. of extractives were left from the first two 6 
hour periods and 9.7 gm. from the last. They were united in 
alcohol-water solution and evaporated on dextrin. Final weight 
of dry material, 165.1 gm. 
The dextrin which carried the alcoholic extracts was trans- 
ferred to a small Soxhlet apparatus and extracted with benzene 
successively in a 6, 62, and an 8 hour period. The extract from 
the first two periods weighed 25.39 gm., from the last period 0.61 
gm. They were united, dried at 37°C., and ground up without 
evaporating on dextrin. This was fed to Lot 953 A, Period 3, 
Chart 1, and to Lot 953.B, Period 4, Chart 2. 
The residue from the benzene extraction (alcohol-soluble, ben- 
zene-insoluble material) was made up to 200 gm. with dextrin. 
This preparation was fed to Lot 953 B, Period 2, Chart 2. It 
was later moistened with distilled water and heated 40 minutes 
in an autoclave at 15 pounds’ pressure. 
Chart 2. Lots 953 B, 994 A, and 994 B. 
Lot 953 B. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. ° 
Period 2.—Ration: Same as Period 1 plus the portion of raw beans, 
soluble in alcohol but insoluble in benzene, equivalent to 30 per cent of 
beans. 
