E. V. McCollum and N. Simmonds 63 
In the following charts, the diet in Period 1 consisted of purified 
foodstuffs plus 5 per cent of butter fat. 
COTE SR GUE a a 18.0 
op ly bis qipbesis hoi me ><  ON) ae 3.7 
US OTIS, Oe a ee pe Sr ee 2.0 
Ja ES Te eee ie ean er Se Re |) oe cohen Psa WS: 
POLO Se Pah ED RE ES ne eee a ee eae 5.0 
Pee Oe NI TOUS Merten ee oh. hide ee a ba ca eden ce be 0.266 
Na H3PO..H.0 Ahi. Mo48 26 Go E88) oy ch be Ann eee cy ee er 0.347 
“eb ig 2 ok 2 i 8 rr ae oe .0 954 
CaH.(PO;)2.H2O Mee EOC sen steel sas ore esos: cose) a 2k fey e eh aera Aad fee a eaaee 0.540 
YES URES, a Sk I ees: 2 he rrr 0.118 
anette RE ee oa deve dled aa ebd 1.300 
Chart 1.2 Lots 958 A, 952 A, and 952 B. 
Pe ‘ 
Lot 953 A. Period 1.—Ration: Purified food mixture plus 5 per cent of 
butter fat. 
Period 2.—Ration: Same as Period 1 plus the benzene extract of ether- 
extracted raw navy beans equivalent to 30 gm. of beans per 100 gm. of 
ration. 
Period 3.—Ration: Same as Period 1 plus the benzene extract of the 
alcohol-soluble part of ether-éxtracted raw beans, equivalent to 30 gm. 
of beans per 100 gm. of ration; 7.e., alcohol-soluble,? benzene-soluble part 
of the bean. 
This experiment shows that the water-soluble B is extracted 
from raw beans by hot alcohol, but not by ether or warm ben- 
zene. After the substance has been extracted from beans by 
means of alcohol, it is, however, soluble in benzene (see Chart 
2, Lot 953 B, Period 4). 
The curves presented in this and the following charts are actual 
records of animals, and are representative of a group usually of four in- 
dividuals. For economy of space a single record is shown. The broken 
curves marked N represent the normal expectation of growth. 
3 Only 95 per cent ‘alcohol was employed in making the alcoholic ex- 
tracts used in these experiments. 
