46 Gas Analysis. V 
The method employed consisted in first filling the diffusion tube 
and burette with pure CO, gas from a Kipp generator. The rub- 
ber stopper was then inserted in the diffusion tube. The tube was 
immersed in water at various temperatures and the volume of gas 
read on the burette. 2 cc. of diluted acidified blood entirely 
freed from CO: were injected through the stopper into the diffusion 
tube. The tube was thoroughly shaken at various temperatures 
and the volume of gas going into solution was read from the 
burette. In the calculation allowance was made for the fact that 
the gas in the burette remained always at room temperature. ‘The 
curves in Fig. 4 give the relative distribution of CO, at tempera- 
tures between 12° and 30°C. in gaseous form and in solution. 
BIBLIOGRAPHY. 
. Barcroft, J., and Haldane, J. 8., J. Physiol., 1902, xxviii, 232. 
. Henderson, Y., and Morriss, W. H., J. Biol. Chem., 1917, xxxi, 217. 
. Van Slyke; D.D.; J. Biol. Chem, 1017 ixkx, 304: 
. Henderson, Y., J. Biol. Chem., 1918, xxxiii, 32. 
. Brodie, T. G., J. Phystol., 1909-10, xxxix, 391. 
oF WON Fe 
