W.G. Lyle and L. J. Curtman 5 
TABLE 1. 
10 Cc. Portions Containing 1 Gm. of Stool. 
Amount of 
No. Blood solution. blood in 1 gm. Test. 
of stool. 
cc. 
PEOED CGOPOOlUtION A... co: «sss smces « 0.005 Blue. 
oe ie : LOIRE. oh. eee eS | 0.001 Greenish blue. 
Seeieo ss SOEs yt eas 0.001 a ie 
A OLoes y BREN MoO 0.0005 Light green. 
pees 1 se Bast. «fete 0.0001 o ¥ 
Gen O° tt a OOF ea eee Slee .... | 0.00005 Negative. 
fee Oslin. . Die ae tt oe Ste 0.00003 
SMM OTILOOU Gs sr cats nse ecm cles occ 0.0 haa 
tests were obtained in extracts which gave unmistakably positive 
results with the usual quantity of perhydrol. 
To determine whether any advantage was to be gained by 
boiling the stool with acetic acid before extraction, the following 
experiments were made. Four portions, of 9 to 10 gm. each, 
of a negative, formed stool were treated as follows: 
(a) To one portion 25 cc. of distilled water were added. (6) 
To another portion 25 cc. of water containing 5 cc. of glacial acetic 
acid were added. (c) Same as (a) with the addition of 1 cc. of 
blood Solution A. (d) The same as (6) with the addition of 1 
cc. of blood Solution A. All were boiled for 10 minutes and the 
regular procedure was applied with the following results: 
SP Ae, . LOIN ED che ine Sea Dw als wad siele Negative. 
b “cc 
fT M5 G5 sis swash ha muowrred be Mons heat me ws Positive. 
d Hl “c 
The above experiments were repeated with the same results, 
showing that the preliminary boiling of the stool with acetic acid 
and water possesses no advantage over the adopted procedure 
of boiling first with a little water and subsequently extracting 
with acetic acid and ether. 
An examination of the various foods and pills given to patients 
in the hospital was made to see if they gave the test for blood. 
The procedure was the same as that employed in the examination 
of stools. For.analysis about 25 gm. of each food were taken and 
