208 Thyroid and Adrenal 
lard diet. Two series were used for thyroid feeding, the third 
one serving as control. Each rat was kept in a separate cage. 
As thyroid administration 0.1 gm. of desiccated thyroid per day 
was prescribed to each rat of the one series, and this was contin- 
ued for 23 days. Every rat of the other series received 1 gm. of 
desiccated thyroid per day for 4 days. Thyroid powder, mixed. 
with two or three times the weight of the dog biscuit-lard paste, 
was readily eaten. After this had been consumed, the paste was. 
given freely. At the end of the period, the animal was bled and 
both adrenals were carefully freed from the surrounding tissues 
and weighed together. The epinephrine content was determined 
by Folin’s method,. modified for smaller quantities of material. 
The protein of the ground adrenals was precipitated by acid and 
salt in a10-cc. volumetric flask. The mixture was diluted up to the 
mark and filtered. 8 cc. of ‘the filtrate were taken into a 25 cc. 
volumetric flask and the color, produced in this flask, was com- 
pared with the standard color in another 25 ce. flask. For the 
standard color, two 25 cc. volumetric flasks containing 0.2 and 
0.4 mg. of uric acid, respectively, were provided and the flask 
which was the nearer in color to the epinephrine flask was used. 
The results are shown in Tables I and II. 
TABLE I. 
The Epinephrine Content of the Adrenals of Normal Rats. 
Weight of 
Weight of ae Epineph- | Epineph- Epineph- 
Rat, | Sex | welate. | gf, | 100m. of | Bein ie re wer e-em. of body 
Heights ees 
gm. mg. mg. mg. mg. mg. : 
I M. 304.8 36.9 12,1 0.102 pay gi 0,033 
II sé 269.7 35.3 ie 0.094 2.66 0.035 
III ‘fe 254.3 63.1 24.8 0.162 2.57 0.064 
IV oe 218.1 57.5 26.4 0.099 1:¥2 0.045 
V a 180.0 33.8 18.8 0.091 2.69 0.051 
VI F. 243.8 50.6 20.8 0.134 2.65 0.055 
it BO aaa 240.2 50.6 211 0.174 3.44 0.072 
VIII Af 199.4 39.5 19.8 0.098 2.48 0.049 
1,6 o 196.2 39.5 20.1 0.104. 2.63 0.053 
ag« as 194.6 39.1 20.1 0.100 2.56 0.051 
xa as 69.2 25.4 36.7 0.056 220 0.081 
——_— | | —|—— 
eT | 
Average..... 215.5 42.8 21:3 0.110 2.58 0.054 
