won Yeast Nucleic Acid. II 
obtained. In the purification of the brucine salt one may, to 
some extent, be guided by its melting point. The pure material 
on heating in a capillary tube first contracts at 183°C. (corrected), 
then melts, and finally decomposes at 198°C. (corrected). How- 
ever, even after this phase is attained it is advisable to repeat 
recrystallization at least three times. The optical rotation of the 
substance was not measured because of its great insolubility in 
water and in other solvents. The solubility of the salt is greater 
in dilute alcohol than in water, but the solubility even in this 
reagent is not great enough to permit an accurate optical 
measurement. 
The composition of the brucine salt (No. 70) was the following: 
0.1027 gm. substance gave 0.1998 gm. CO» and 0.0584 gm. HO. 
0.2000 “ ef: “* 12.0 ce. of nitrogen at t° = 22°C. and p = 764 mm. 
0.2000 ‘ 2 “0.0168 gm. MgeP207. 
Calculated for 
CssHesNePO7+ 7 H20: Found: 
sg ee De oe Bee ew ce ee ee 53.20 53.06 — 
Leos witha ard elem neve ahah ie 5a 8 0k ee oa ee 6.51 6.37 
IN Sete eStart eet Pee oe Oe eee eee 6.80 6.96 
Pyro accBiok ce Bele at let ke hee eee nee 2.52 2.37 
Conversion of the Brucine Salt into the Barvum Salt—The bru- 
cine salt was dissolved in hot water by means of an excess of 
ammonia water, and the brucine was extracted by means of 
chloroform in a separating funnel. The aqueous solution of the 
nucleotide was repeatedly evaporated to dryness under dimin- 
ished pressure with an excess of barium hydroxide until all 
ammonium was removed. The residue was then dissolved by the 
aid of some sulfuric acid, and to the solution a barium hy- 
droxide solution was added until the reaction turned slightly 
alkaline to phenolphthalein. The filtrate from the barium 
hydroxide was concentrated under diminished pressure at a tem- 
perature of the water bath not exceeding 50°C. It is preferable 
to interrupt the distillation just before the nucleotide begins to 
settle out while the distillation is still in progress, since in such 
case it may have an amorphous appearance. If desired the 
barium salt may be recrystallized out of water. When air-dry 
the substance has a granular appearance, and microscopically it 
consists of rosettes composed of long needles, as is shown in the 
figures. 
