C. K. Watanabe 257 
administered the following symptoms appeared almost without 
exception, though usually not all in the same rabbit: dyspnea, 
depression, emaciation, twitching of head and legs, continuous 
jerking of head backwards and forwards, chattering of the teeth, 
fine tremor of the muscles, fibrillous tremor of extremities, opis- 
thotonus, nystagmus following convulsion, extension of the hind 
legs, diarrhea, salivation, and disturbance of equilibrium. 
Hypoglycemia after Injectton of Guanidine Hydrochloride. 
At first we administered 0.15 gm. per kilo of body weight to 
Rabbit 7 and 0.2 gm. per kilo of body weight to Rabbit 8 at 9.10 
a.m. and kept them both under observation until the following 
day. The first few hours samples were taken every hour and later 
TABLE II. 
Hypoglycemia after the Injection of Guanidine Hydrochloride. 
: g : Blood sugar. 
i=) GS 
Z |e 
Sete eae hse |) os te). a Saleece |) eG ubn eh uid sae 
= 3 |3o}] 4 a a S fad fo} fo} a a, at 4, 
me ee wee eee eet | ty he ett 19 am.) 
1917 Mic Ade ee ale Bee ie t bent oe t ae foe es cent Het 
7 | June 30} 2,220/0.15/0.101/0.100)0.122/0.124/0. 1100/0 .098)0 .085|0 .077|0 .073)/0 .099)0 .095 
8 | July 2/2,240)0. 20/0. 121|0.112/0.125.0.127|0.108)0.111/0.100/0.101 0.054/0.081 
at intervals of a few hours. In Rabbit 7 mild hypoglycemia was 
first observed 5 hours after injection and continued. until the 
next morning, whereas No. 8 first showed a marked hypogly- 
cemia at 8.10 p.m. which continued until the next morning when 
the rabbit died in convulsions. 
The following group of rabbits was used to confirm the above 
phenomena, the blood samples being drawn at intervals of a few 
hours in this case. Twelve out of twenty-one showed hypogly- 
cemia, some of them showing a reduction of more than half the 
normal blood sugar. Three cases were suspected but six cases 
show negative results. We later confirmed the fact that in these 
negative cases the dose was not large enough to induce hypogly- 
cemia. The urine of the rabbits of this group was tested but none 
showed any sugar. In most cases hypoglycemia appears 7 hours 
