C. K. Watanabe 259 
proportional to the dose of the drug administered. Rabbit 23, 
however, received the largest dose and this died before show- 
ing hypoglycemia. In the successive samples of blood from each 
rabbit we notice that in the first and'second samples after injec- 
tion of the drug the sugar content shows an increase over that in 
the sample taken before injection. 
On the surviving rabbits a second injection of a little larger 
dose than the first was performed after time had been allowed 
for recovery from the first injection. In all cases except Nos. 
TABLE IV. 
Second Injection of Guanrdine. 
: 5} g Blood sugar. 
z oy Ss ae 
2 Date. Weight. = 3 : 3 F F g F aes 
= Sc5 2 3 3S Q Q a at 
aa Cle oo ie = ee re 5 9 a.m 
1917 gm. gm. |per cent)per cent|per cent\per cent|per cent per cent|per cent 
1 | July 8) 1,880} 0.25 | 0.111} 0.142) 0.154) 0.140} 0.120) 0.096) 0.123 
7 “~~ 10} 2,080 | 0.30 | 0.109} 0.196) 0.171) 0.173} 0.150) 0.096) 0.096 
9 a 14) 2,260 | 0.25 | 00152 0.125; 0.091) 0.095} 0.098) 0.1438 
11 16} 1,800 | 0.25 | 0.125) 0.143) 0.104) 0.099) 0.095} 0.095) 0.125 
12 31) 1,280 | 0.25 | 0.111} 0.102) 0.093) 0.104] 0.111} 0.087 
14| Aug. 2) 1,440 | 0.30.| 0.103 0.049 
16 - 3| 1,320 | 0.20 | 0.1386) 0.176) 0.170} 0.171) 0.136} 0.101) 0.116 
17 Se 4| 1,520 | 0.20 | 0.127| 0.137) 0.154) 0.181) 0.124) 0.120) 0.125 
18 “¢  - 12} 1,220 | 0.380 | 0.109 0.074 . 
19 ** 18) 1,400 | 0.25 | 0.107 0.154) 0.125) 0.122) 0.107| 0.111 
20 vue ane 1G) 1,620 |.0:20%|..0 
.108 0.150} 0.127) 0.122) 0.106) 0.088 
14 and 18 there was no hypoglycemia—the exceptions showing 
hypoglycemia just before they died in convulsions. 
In consideration of these facts we may say that all rabbits will 
also show hypoglycemia after injection of the second dose of the 
drug, if the dose is large enough, though in this case they will 
die. It is interesting to note that the second injection of a large 
dose of guanidine provokes, in most cases, a hyperglycemia a 
few hours after administration and it is more marked than that 
appearing after the first injection. In these animals there is no gly- 
cosuria and most of them died the following night in convulsions. 
A third injection was performed on the survivors of the second 
injection after an interval of 1 or 2 weeks. Of these only No. 11 
THE JOURNAL OF BIOLOGICAL CHEMISTRY, VOL. XXXIII, NO. 2 
