A. L. Daniels and R. Loughlin 297 
containing no butter fat, grew at about half the normal rate and 
finally died unless butter fat was given (Chart III). The prompt 
recovery of the surviving animal (Rat 8) when 5 per cent of butter 
fat was added indicates that the lack of the fat-soluble food ac- 
cessory was the inhibiting factor in this diet. The animals 
receiving the 2 per cent butter fat ration made somewhat better 
growth than those receiving none. Their curves of growth, how- 
ever, are below those of normal animals (Chart II). Three females 
of the group reproduced (Chart I, Rats 6, 12, and 13). Their young 
were alsoundersized. Irritable dispositions and marked roughness 
of coats characterized all the animals of this group.. Such con- 
ditions indicate malnutrition. The change from the 2 per cent 
butter fat ration to a 5 per cent ration stimulated growth, and 
reproduction quickly followed in the animals which had not pre- 
viously reproduced (Chart III, Rats 7 and 14). 
Since all the animals receiving the 5 per cent butter fat ration: 
were normal in every respect, proof is furnished for the presence 
of a considerable amount of the water-soluble food accessory in 
the diets. When the peanuts formed 56 per cent of the ration, 
there was sufficient water-soluble B for the experimental animals. 
A comparison of the inorganic content of the peanut and the 
soy bean shows the peanut to be even poorer than the soy bean 
in certain respects; namely, calcium, potassium, magnesium, and 
sulfur.4 Therefore since the mineral content of the soy bean 
had been found lacking,> various amounts of suitable minerals 
were added to make the mineral content of the peanut rations 
similar to that of milk, which previous investigations have dem- 
onstrated to be adequate for normal growth of rats. On the 
other hand, peanut meal,’ which includes a considerable propor- 
tion of hulls, apparently contains sufficient amounts of these 
necessary inorganic constituents for physiologic well being. Rats 
fed a mixture of peanut meal furnishing 18 per cent of protein, 
5 per cent of butter fat, lard, and cornstarch, to which no min- 
erals were added, grew normally, reproduced, and successfully 
reared their young (Chart II, Rats 27, 28, and 29). 
4 Forbes, E. B., Beegle, F. M., and Mensching, J. E., Ohio Agric. Exp. 
Station, Bull. 255, 1913. 
6 Daniels, A. L., and Nichols, N. B., J. Biol. Chem., 1917, xxxil, 91. 
6 Alabama Agric. and Ind. Dept., Bull. 76, 1916. 
