396 Ascaris suum 
the attack of the digestive enzymes. It should be noted that he killed 
the worms by electrolysis, a method by which enzymes may be destroyed 
(Burge and Burge, 1915, 6). 
In addition to these interesting facts another group of phenomena 
has accumulated. It has been shown (Burge, 1916) that the catalase 
content of muscles is highest in those in which more work is done, 7.e. 
in which more oxidation is taking place; that during starvation the cata- 
lase content of the fat and muscles, with the exception of the heart, is less 
than normal (Burge and Neill, 1917); that the catalase content of the 
liver, heart, and blood is decreased in phosphorus poisoning and that the 
tissues are autolyzed in the same proportions that they lose catalase 
(Burge, 1917). 
It occurred to the author that if this theory was valid and the 
presence of oxidative processes in the intestinal parasites pro- 
tected them from digestion, then the body walls of these para- 
sites should yield comparatively large amounts of catalase, if the 
amount of catalase in the tissue was an indication of the oxida- 
tive processes. The common Ascarzs, a roundworm, from the 
hog was used in a series of experiments to determine the amount 
and distribution of catalase in the body. 
The method used was one adapted from ordinary gas analysis. 
It seems to have advantages over the one used by Burge (1916) 
in that the gas may be collected under standard conditions, the 
apparatus is easier to handle, is neater, and perhaps more ac- 
curate, although it must be borne in mind that the determination 
is not an absolute one but comparative. The text-figure shows 
the apparatus. The material was washed with a 0.75 per cent 
sodium chloride solution and finely chopped up, weighed in a 
crucible, and introduced into the bottle C in which had been put 
25 ec. of- one-half diluted commercial hydrogen peroxide. The 
pinch-cock E was opened and the bottle stoppered as indicated 
in. the figure, connecting the bottle to the burette, which was 
filled with water. The stop-cock B was then opened and the 
water in the burette D leveled to the zero mark by raising or 
lowering the bulb A. When this was done the pinch-cock was 
closed, the crucible upset in the hydrogen peroxide, and the bottle 
C was shaken for 10 minutes. At the end of that time the stop- 
cock B was closed and the water in the leveling bulb brought to 
the level of the water in the burette and the reading made. ‘This 
was corrected for 760 mm. pressure and 25°C. It is obvious 
