eA Gecnln 409 
While we know that this excess of unsaturated fatty acids has a 
hemolytic action, and no doubt intensifies the hemolysis, yet it, 
cannot be the primary cause, as we found in Cases 8, 138, 14, 19 
(Tables I and II) higher unsaturated fatty acids in even larger 
quantities than in normal blood with normal red blood count and 
hemoglobin content. 
The iodine values of fat plus lipoid substances show small 
variation in pathological cases as well as in normal cases, and 
have neither qualitative nor quantitative significance. A higher 
iodine number does not mean that there are present more highly 
unsaturated fatty acids than oleic, or that the proportion of 
unsaturated to saturated is larger. 
SUMMARY. 
Unsaturated fatty acids are a product of normal metabolism, 
being present in normal human blood, in which they form 48.0 
per cent of the total fatty acids. Judged by their iodine absorp- 
tion power there are, in addition to oleic acid, other unsaturated 
fatty acids both higher and lower than oleic, although in small 
amounts. 
The iodine numbers of the unsaturated fatty acids in patho- 
logical conditions are generally higher than in normal individuals, 
especially in cases with low hemoglobin values. As such acids 
exist in normal human blood as well as in pathological conditions 
without anemia, it is necessary to look further for the primary 
cause of toxic hemolysis. 
The Gephart-Csonka method for fatty acid determination in 
feces has been applied to blood in the present work, thus avoiding 
the time-consuming extraction common to other methods. 
