420 Pellagra-Producing Diets. V 
response. Chart 2, Lot 1,021, showed that two purified food 
additions, protein and fat-soluble A, do not enhance the diet so 
as to support growth. 
The growth of these animals was not so rapid as they are ca- 
pable of making when the diet is highly satisfactory. This is 
illustrated by the records of Lot 1,024, Chart 4, when compared 
with Lots 1,022 and 1,023, Chart 3. In the latter the best rats be- 
came stunted before reaching fulladult size. These records make 
it clear that the diet of peas, wheat flour, and cottonseed oil can 
‘be supplemented with well known dietary components so as to 
induce a moderate amount of growth in the rat. It is necessary 
to conclude, therefore, that the dietary deficiencies of this food 
mixture, which has induced in dogs symptoms closely resembling 
pellagra in man, are not in the nature of unidentified ‘‘ protec- 
tive’ substances, except that there is in some degree a shortage 
of the content of fat-soluble A. Sodium chloride and calcium 
carbonate, together with purified protein and fat-soluble A, 
render the diet practically complete (Chart 4). 
Lot 1,023.—These records illustrate the fact that the protein 
mixture derived from peas and patent flour (12.1 per cent) is 
adequate for the support of a good rate of growth (Period 2) — 
after a period of stunting for 5 weeks. ‘These records further 
support the view that the first limiting factor in this food mix- 
ture is the quality of the inorganic content (see Charts 1 and 2). 
These growth curves are not of so steep a gradient, however, as 
can be secured by the further improvement of the protein content 
of the diet by the addition of purified casein (Chart 4). A com- 
parison of Charts 3 and 4 makes it evident that more fat-soluble 
A must be added before the optimum growth can be secured with 
this ration. 
Chart 4. Lot 1,024.—These records show the degree of improve- 
ment of a mixture of peas, patent flour, and cottonseed oil, which 
can be effected by the addition of three recognized dietary factors, 
protein, salts, and fat-soluble A. Not only is the growth on the 
diet supplemented in this way nearly at the maximum rate, but 
three litters of young have been secured. 
A mixture of peas, patent flour (crackers), and cottonseed oil 
induced in dogs a profound state of malnutrition, characterized 
by sore mouth, sloughing of the mucosa, diarrhea, infection of the 
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