P. A. Levene 427 
containing barium hydroxide and decomposed by means of hy- 
drogen sulfide. Care was taken to keep the solution slightly on 
the alkaline side. The excess of hydrogen sulfide was removed 
by aeration; the solution was then made distinctly alkaline, fil- 
tered, again neutralized, and concentrated to a volume of 500 ce. 
The barium was then removed quantitatively and from the solu- 
tion the brucine salts were prepared in the usual way. 
The analysis of the mixed brucine salts gave the following value. 
0.200 gm. substance gave 15.4 ec. N at 18°C. and 747.2 mm. 
For cytosine-uracil dinucleotide C;yH25N7P20i15.4(C23H2s5N204) +14 HO 
Calculated: Found: 
Mr tee Wey OF ec il, a gk ee Nee NENT opt ait 8.46 8.88 
This material was recrystallized nine times. The final product 
gave the following analytical results. 
0.200 gm. substance gave 12.4 cc. N at 17°C. and 757.2 mm. 
OeOr2 " “0.1970 gm. CO, 0.0582 gm. H.20O, and 0.0058 
gm. ash. 
Calculated for 
CssHes3 NePO7+7 H20: Found: 
EE ON Bs) EE. Paes 28 ns hy ew dvs 4 PS Dene 53.20 53.08 
eee Re. yh ag de tei eS ane 4 oe 6.51 6.43 
IN ol vdele leads GO SURDicreCerl Cre ree oes ec cis ae ara 6.80 120 
This brucine salt was then converted into the barium salt. 
The salt crystallized in the manner described in the previous com- 
munication. It was redissolved in a small volume of a 10 per cent 
solution of sulfuric acid. The solution was neutralized with 
barium hydroxide, filtered, and allowed to crystallize at room 
temperature. The figure illustrates the crystals. 
The substance gave the following analytical data. 
0.200 gm. substance required for neutralization 8.92 cc. of 0.1 N acid. 
OsvE2 se gave 0.0912 gm. COs, 0.0230 gm. H20, and 0.0514 
gm. ash. 
Calculated for 
CoH: N2O3sPBa: Found: 
(25 lb RE iar eg aR Sa Se 23.50 23.64 
1 a Ca IRS Soa i ae eng) 2.41 2.45 
1S eg RRA Oy 7, Seagal ema ae ee 1 6.10 6.24 
Bee Or eas. ae cust Oe est he 48.97 48.86 
The optical rotation of the substance was the following: 
[or] Lone 0.14° X 100 Ny 
RS 
he Lx 4 i 
