* 
456 Enzyme Action 
The results of check determinations in this experiment were a 
little irregular, but the tubes containing 1 part of bromate in 
100,000 showed a significant increase in digestion. 
Experiment II.—June 16, 1917. 2 gm. of casein, 0.4 gm. of Merck’s 
trypsin, per 100 ce. of solution. Digestion period, 43 hours. 
Titration of 50 ec. 
Increase in amino- 
acids during diges- 
tion. “Ces! 0:2. -N 
NaOH to neutra!- 
ize. 
Solution. 
Buffer + casein + trypsin. 5.9% Dnt 
6.2 
‘ ‘<6 ‘< + KBrO; 1: 10,000 | 6.4 6.1 
5.8 
Ree 
‘ «6 ae “1: 50,000 5.9 5.9 
6.0 
6.6 
73 “ 6c“ 73 1: 100,000 632 Svs 
6.0 
6.4 | 
‘ « as fe Le8200,006 6.7 6.8 
tee: 
Gaz 
“ ‘ ‘ “ 1: 300,000 6.20 
5.8 
« « « =e L500, 000 6.0 5.6 
The results of this experiment again show considerable indi- 
vidual variations, but with higher amino-acid contents at bro- 
mate concentrations of 1 in 100,000 and 1 in 200,000, the latter 
being most marked. 
