I. 8. Falk and C.-E. A. Winslow 457 
Experiment I1I.—June 29, 1917. 2 gm. of Merck’s casein, 0.1 gm. of 
Fairchild Brothers and Foster’s trypsin, per 100 ce. Digestion period, 34 
hours. 
Titration of 50 ce. 
: Increase in amino- 
Solution. _ acids during diges- 
tion. Ce. 0.2N 
NaOH to neutral- 
ize. 
Buffer + casein + trypsin. 
bo bo bo 
oo ff 
aD 
— 
‘ «“ és + KBrO;1: 10,000 
bo bo bo 
“In 
i) 
~] 
Ww Ww 
Ww Ww 
(o) 
oo 
“ce << “cc 6c 1 : 50,000 
et) 
WwW &w 
kt OD 
(se) 
= 
¢é 73 (T3 (73 il : 100,000 
OO 
fer) 
i=) 
: ‘ Sp ert 150,000 
Hq Or pe 
lm 0 
ae 
cn 
Hm bo 
| oop) 
Go 
ea 
sé Clr" “c cc it : 200,000 
[s) 
on 
bo 
aJ 
bo 0 
SS iss) 
77 73 73 cc 1: 500,000 
bo 
Or 
In this experiment the digestion increases regularly with de- 
creasing amounts of bromate to a concentration of 1 part in 150,000 
and then decreases again with further dilution of the salt. Here 
as in other experiments it appears, however, that even certain 
high concentrations of bromate (1 part in 10,000) are rather 
favorable than otherwise to the process of enzyme action. 
