458 Enzyme Action 
Experiment IV.—July 138, 1917. 2 gm. of Merck’s casein, 0.2 gm. of 
Fairchild Brothers and Foster’s trypsin, per 100 cc. Digestion period, 3 
hours. 
Titration of 50 ec. 
’ |Increase in amino- 
Solution. acids during diges- 
tion. Cc. 0.2 n 
NaOH to neutral- 
1Z€-6 
| Aaill 
Buffer + casein + trypsin. 4.85 ~.75 
Zot 
2.8 
«“ © + KBrO; 1: 100,000 3:0) o30 
3.1 
. 3.25 
te « « “ 1: 120,000 Bak Se 
. 4.4 
3.89 
« re : 1: 140,000 3.3 3.6 
3.6 
4.1 
“ “ “ ee 1: 160,000 4.25 4.15 
4.1 
4,25 
“ « “ 662 1:180;000 4.0 4.15 
4.2 
5.5 
«6 ‘“ « eet 200,000 | 5.8 5.7 
5.8 
4.9 
« « ¢ 4 1: 250,000 45 7420 
4.0 
Again the result is an increase of enzyme action under the in- 
fluence of bromate up to a concentration of 1 part in 200,000. 
In both these last experiments the digestive action was more than 
doubled by the stimulating effect of the bromate. 
